生物学杂志

生物学杂志

• 研究报告 • 上一篇    下一篇

Xanthomonas campestris CCTCC M2015714以甘油为底物发酵产黄原胶的性质分析

  

  1. 1. 江南大学 生物工程学院， 无锡 214122； 2. 江苏瑞光生物科技有限公司， 无锡 214125
  • 出版日期:2017-10-18 发布日期:2017-10-18
  • 通讯作者: 詹晓北，教授，博士研究生导师，主要从事高黏度反应器和微生物多糖的研究，E-mail:xbzhan@yahoo.com
  • 作者简介:王子朝，博士，主要从事微生物多糖的发酵生产及应用研究，E-mail: 6832917@163.com
  • 基金资助:
    国家自然科学基金 (31171640)；中央高校基本科研业务费专项资金项目 (JUSRP51632A，JUSRP51504)

Characterization of xanthan gum produced from glycerol by Xanthomonas campestris CCTCC M2015714

  1. 1.School of Biotechnology, Jiangnan University, Wuxi 214122; 2.Jiangsu Ruiguang Biotech Co. Ltd., Wuxi 214125, China
  • Online:2017-10-18 Published:2017-10-18

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摘要： 主要研究以甘油为底物由Xanthomonas campestris CCTCC M2015714发酵合成的黄原胶，并分析黄原胶的特性。合成的黄原胶中含有葡萄糖、甘露糖和葡萄糖醛酸3种单糖，摩尔比为葡萄糖∶ 甘露糖∶ 葡萄糖醛酸=2.0∶ 1.65∶ 1.0。红外光谱和核磁共振结果显示：甘油产黄原胶的官能团和化学结构与商品级黄原胶基本一致。甘油产黄原胶的分子质量 (3.0×106 u) 约为商品级黄原胶的一半 (6.4×106 u)，且其水溶液的黏度远低于商品级黄原胶。结果展示了甘油产黄原胶的优良特性，同时为甘油的利用提供了一个有效途径。

关键词: 甘油, 野油菜黄单胞菌, 黄原胶, 性质分析

Abstract: This study focused on the xanthan gum produced by Xanthomonas campestris CCTCC M2015714 with carbon source of glycerol. The monosaccharide composition of xanthan gum was glucose, mannose and glucuronic acid, the molar ratio of three monosaccharides was glucose∶ mannose∶ glucuronic acid=2.0∶ 1.65∶ 1.0. The results of FT-IR and NMR showed that xanthan gum produced from glycerol had similar functional groups and chemical structure comparing with commercial xanthan. The molecular weight of xanthan gum produced using our method was 3.0×106 u, which was about half of the commercial xanthan (6.4×106 u). Moreover, the viscosity of xanthan gum produced from glycerol was significantly lower than that of the commercial xanthan. Depending on this work, xanthan gum produced from glycerol exhibited great characteristics and also supplied a valuable method for glycerol application.

Key words: glycerol, Xanthomonas campestris, xanthan gum, characterization