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高碱度和高氨对达里湖瓦氏雅罗鱼耗氧率和排氨率的影响

  

  1. 1. 淡水鱼类育种国家地方联合工程实验室 农业部淡水水产生物技术与遗传育种重点实验室中国水产科学研究院特殊生境鱼类种质特性与抗逆育种重点实验室 中国水产科学研究院黑龙江水产研究所, 哈尔滨 150070; 2. 上海海洋大学 水产与生命学院, 上海 201306
  • 出版日期:2016-12-18 发布日期:2016-12-18
  • 通讯作者: 梁利群,研究员,博士生导师,研究方向为水产动物基因工程育种,E-mail: llq-1019@163.com
  • 作者简介:何强,硕士研究生,专业方向为鱼类分子生物学,E-mail: hqiang215@163.com
  • 基金资助:
    国家自然基金项目 (31461163004);黑龙江省科技攻关项目(GCI2B307)

Effects of high carbonate alkalinity water and high ammonia water on oxygen consumption and ammonia excretion in Leuciscus waleckii Dybowski

  1. 1. National & Local United Engineering Laboratory of Freshwater fish Breeding; Key Laboratory of Freshwater AquaticBiotechnology and Genetic Breeding, Ministry of Agriculture; Key Laboratory of Fish Stress Resistance Breeding and Germplasm Characteristics on Special Habitats, Chinese Academy of Fishery Sciences; Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070; 2.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
  • Online:2016-12-18 Published:2016-12-18

摘要:

采用静水密闭的方法,比较了体质量为(72.62±3.70 )g达里湖瓦氏雅罗鱼(Leuciscus waleckii Dybowski,简称雅罗鱼)在高碱度(NaHCO3 50 mmol/L)和高氨(NH4CO3 1.5 mmol/L)胁迫下处理4个时间点(2 h、4 h、6 h和8 h)耗氧率和排氨率的变化。耗氧率实验结果表明:与淡水组相比,高碱度和高氨显著抑制了雅罗鱼的耗氧率(P<0.05);高碱度组与高氨组相比,雅罗鱼耗氧率除了在监测的第1个时间点内存在显著性差异外(P<0.05),其他时间点无明显差异(P>0.05)。排氨率实验结果显示:与淡水组相比,高碱度和高氨均显著抑制了雅罗鱼的排氨率(P<0.05)。其中高氨组排氨率在所监测的4个时间点内全部下降,并与淡水组和高碱度组相比存在极显著差异(P<0.01)。能量代谢研究发现,淡水组和高碱度组氧氮比均无显著差异(P>0.05);与淡水对照组和高碱度组比较,高氨组氧氮比差异极显著(P<0.01)。

关键词: 瓦氏雅罗鱼, 碳酸盐碱度, 耗氧率, 排氨率, 氧氮比

Abstract: Using hydrostatic water in sealed chamber for measurement, this study compared the change of oxygen consumption rate and ammonia excretion rate of Leuciscus waleckii Dybowski (72.62±3.70)g exposed in high carbonate alkalinity water (CA 50 mmol/L) and high ammonia water (NH4CO3 1.5 mmol/L) for 4 time points (2 h, 4 h, 6 h and 8 h) measurement. Oxygen consumption rate was significantly inhibited in carbonate alkalinity water and high ammonia water when compared that in fresh water (P<0.05). No significant difference of the oxygen consumption rate between carbonate alkalinity and ammonia water treated fish was detected for the last three tested time points, 4, 6, and 8 except for the first testing time point. Results of ammonia excretion rate showed that compared with those in fresh water, the ammonia excretion rate of fish exposed in carbonate alkalinity water and ammonia water was significantly inhibited (P<0.05). In ammonia water, the ammonia excretion rate of L. waleckii Dybowski for all 4 time points decreased, which had an extremely significant difference compared with fresh water and carbonate alkalinity water. Energy metabolism (O∶ N) study found that there had no significant difference in oxygen-nitrogen ratio in fish between fresh water and carbonate alkalinity water. However, compared with fish in fresh water and carbonate alkalinity water, fish in ammonia water had an extremely significant difference in oxygen-nitrogen ratio. 

Key words: Leuciscus waleckii Dybowski, carbonate alkalinity, oxygen consumption rate, ammonia excretion rate, oxygen-nitrogen ratio