生物学杂志

生物学杂志

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D-半乳糖致糖基化小鼠模型的评价

  

  1. 宁波大学 海洋学院 应用海洋生物技术教育部重点实验室
    浙江海洋高效健康水产养殖协同创新中心， 宁波 315211
  • 出版日期:2016-10-18 发布日期:2016-10-18
  • 通讯作者: 宋微微，讲师，研究方向为水产动物遗传育种，E-mail: songweiwei@nbu.edu.cn
  • 作者简介:冀雄飞，硕士，研究方向为动物遗传育种研究，E-mail:jixiongfei821@163.com
  • 基金资助:

    国家自然科学基金项目（41206114，41176124）；浙江省教育厅项目（Y201224605）；浙江省自然科学基金项目（LQ12C19002）；浙江省自然科学基金重点项目（Z3110482）；宁波大学“水产”浙江省重中之重开放基金

Establishment and evaluation of glycation model induced by D-galactose in mice

  1. Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, School of Marine Schences,Ningbo University, Ningbo315211, China
  • Online:2016-10-18 Published:2016-10-18

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摘要：

采用200 mg·kg-1·d-1的D-半乳糖诱导小鼠45 d建立糖基化模型，通过新物体识别检测模型组与正常对照组小鼠行为学差异，进一步利用酶联免疫法检测小鼠脑、心、肝、脾、肺、肾、皮肤、肌肉和血清中的糖基化末端产物和相关受体的表达量（AGEs、RAGE、sRAGE和esRAGE），分析该模型中4种指标的变化情况。结果表明模型组小鼠的辨别指数显著低于空白组（P<0.01），模型组小鼠各组织器官中的糖基化末端产物和相关受体的表达量显著高于空白组（P<0.01）；D-半乳糖慢性处理45 d后的小鼠从行为上有一定的迟缓，同时在对各个不同组织器官的生理指标的检测中发现机体处于高糖基化状态，可用于研究AGEs及其受体的发病机理和药物治疗作用途径，并且可以选用多个组织器官作为实验靶点。旨在为颈背部皮下注射D-半乳糖致糖基化小鼠模型提供理论依据。

关键词: D-半乳糖, 糖基化模型, AGEs, RAGE, sRAGE, esRAGE

Abstract:

To provide a theoretical basis of a new rat model of glycation, the mice of glycation were induced by injecting D-galactose (200 mg kg-1 d-1) using injecting method for 45 days, which were measured the behavioral differences from model group to others and detected the index related to the glycation as AGEs, RAGE, sRAGE and esRAGE in variety organs of mice ( as brain, heart, liver, spleen, lung, kidney, skin, muscles and serum). The results showed that the DI of model group was decreased compared with that of the control group (P<0.01), though the number value of AGEs, RAGE, sRAGE and esRAGE of each tissues in mice were higher than that of control (P<0.01). This experiment investigated that it could make organism in a state of high glycosylation when they were injected by D-galactose (200 mg kg-1 d-1) for 45 days. The model could be used in the study of the etiopathogenesis of AGEs which could discover the receptors and the pathway of pharmaceutical drug. And the index changing form in multiple tissues would give the researchers some suggestions in experimental targets using the model.

Key words: D-galactose, glycation model, AGEs, RAGE, sRAGE, esRAGE