生物学杂志
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摘要： 从环境土壤中筛选到一株酶活较高、高温耐碱性的果胶酶菌株AP-1并对其产酶性质和分离纯化做了初步研究。酶活性质研究结果表明：该酶最适温度为70℃，最适pH 7.5。稳定性实验表明：该酶具有较好的耐碱性，同等条件下Mg2+对该酶具有显著的激活作用。分离纯化实验表明：硫酸铵盐析法中80%饱和度的硫酸铵溶液的纯化倍数最高达到了2.20倍；有机溶剂沉淀法中乙醇（2∶ 1）的纯化效果最好达到了5.20倍。
关键词: 碱性果胶酶, 酶学性质, 分离纯化
Abstract: A bacterial strain producing high-activity and high-temperature alkaline pectinase was obtained from the soil and was named as AP-1. The characteristics and the separation and purification of the pectinase produced by AP-1 were preliminarily studied. The result of the study on pectinase activity showed that the optimal temperature and pH of the pectinase were 70℃ and 7.5, respectively. The experiment of stability showed that the enzyme had good resistance to alkali and was activated remarkably by Mg2+ under the same conditions. The separation and purification showed that the purification effect was better when the enzyme was treated by 80% saturation of ammonium sulfate solution than other, but the purification effect was the best when the enzyme was treated by ethanol.
Key words: alkaline pectinase, enzymatic properties, separation and purification
谭秀华. 高温耐碱性果胶酶菌株的筛选及其产酶的分离纯化研究[J]. 生物学杂志.
TAN Xiu-hua. Isolation of alkaline pectinase-producing strain and the research on separation and purification of its pectinase[J]. .
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