生物学杂志

生物学杂志

• 研究报告 • 上一篇    下一篇

中华芦荟内生真菌抑菌活性研究

  

  1. 重庆工贸职业技术学院生物化学工程系
  • 出版日期:2016-04-18 发布日期:2016-04-18
  • 作者简介:李玲玲，研究生，讲师，主要研究方向为微生物制药，E-mail：729005755@qq.com
  • 基金资助:
    重庆工贸职业技术学院自然科学项目（编号：ZR201310）

Study on antimicrobial activityofendophytic fungi from Aloeveral.var.chinesis(haw.)

  1. Biochemistry Engineering Department, Chongqing Industry&Trade Polytechnic, Chongqing
  • Online:2016-04-18 Published:2016-04-18

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摘要： 从中华芦荟的根和叶中分离出8株内生真菌，其中5株来自叶部，3株来自根部，将分离到的内生真菌对4种指示菌枯草芽孢杆菌Bacillus subtilis、大肠埃希菌Escherichia coli、金黄色葡萄球菌Staphylococcus aureus和啤酒酵母Saccharomyces cerevisiae进行抑菌试验，发现叶部和根部对各有一株内生真菌对3种指示细菌表现出抑菌活性，且叶部的内生真菌对啤酒酵母也有抑制效果。经形态学鉴定，这两株内生真菌都属于黑曲霉。

关键词:  

Abstract:  

Eight strains of endophytic fungi were isolated from the leaf and root of Aloeveral.var.chinesis(haw.), in which, five from the leaf and three from the root. The antimicrobial activity from endophytic fungi was investigated by using the agar diffusion method and the cylinder plate method with Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Saccharomyces cerevisiae as indicator microorganisms. The results showed that two of endophytic fungi which one was isolated from the leaf and the other from root had inhibitory activities to Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and the endophytic fungi from leaf showed antimicrobial activity to Saccharomyces cerevisiae. They were classified as Aspergillus Niger by their morphological features.

Key words: endophytic fungi; aloeveral.var.chinesis(haw.), antimicrobial activity