生物学杂志

生物学杂志

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茉莉酸甲酯对苋菜中苋红素积累的影响

  

  1. 南京农业大学生命科学学院
  • 出版日期:2016-04-18 发布日期:2016-04-18
  • 通讯作者: 甘立军，副教授，从事植物激素生理生化研究,E-mail:ganlj@njau.edu.cn
  • 基金资助:
    国家自然科学基金项目（30300216）

Inductive effect of methyl jasmonate on amaranthin accumulation in Amaranthus mangostanus L. seedlings

  1. College of Life Sciences, Nanjing Agricultural University, Nanjing
  • Online:2016-04-18 Published:2016-04-18

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摘要： 苋红素是一种重要的次生代谢物质，其合成受到光照等因素的诱导。分别以苋菜（Amaranthus mangostanus L.）子叶以及四叶期幼苗为材料，研究了茉莉酸甲酯（methyl jasmonate, MeJA）在苋红素积累中的作用。结果表明：0.02~20 mg/L MeJA在光照及黑暗下均能显著促进苋菜子叶苋红素的积累，且具有明显的浓度效应。在光照下20 mg/L MeJA处理所诱导的苋红素含量是对照组(光照)子叶苋红素含量的2.23倍，同时是黑暗下不加MeJA的对照组的10.91倍。对苋菜幼苗而言，茉莉酸甲酯可以促进苋菜幼苗中苋红素的积累，1.25~10 mg/L范围的茉莉酸甲酯均有效促进了苋红素的积累，且10 mg/L 作用效果最显著。但高浓度的茉莉酸甲酯会抑制苋菜幼苗的生长及叶片的展开。进一步的研究发现，MeJA可引起酪氨酸酶活的上升进而促进苋红素的合成。综上所述，茉莉酸甲酯可以促进苋菜子叶以及幼苗叶片和茎秆中苋红素的积累，但同时对于苋菜幼苗的生长有一定的抑制效应。

关键词: 苋菜, 茉莉酸甲酯, 机械伤害, 苋红素

Abstract:  

Amaranthin is one of the important plant secondary metabolites. Light can trigger the initiation of amaranthin biosynthesis in cotyledons of Amaranthus mangostanus~20 mg/L) induced amaranthin accumulation was dose-dependent both in light and darkness. When in darkness, 20 mg/L MeJA induced 10.91–fold amaranthin contents that of controls. While in light, presence of 20 mg/L MeJA induced 2.23-fold that of cotyledons exposed to light only. When used 4-leaf stage seedlings as material, MeJA (1.25~10 mg/L) could promote amaranthin accumulation both in cotyledons and leafs, stems of Amaranthus mangostanus /Lwas the best. But high concentrations of MeJA could inhibit growth of the seedlings and leaf expansion. The following experiments indicated that MeJA could induce amaranthin acculation by promoting the activity of tyrosinase. From above, MeJA could promote amaranthin accumulation both in seedlings of Amaranthus mangostanus L., but application of MeJA had inhibitory effect on plant growth and development.L. Effect of 10 mg L. In this paper, with Amaranthus mangostanus L. as the material, the effect of MeJA (Methyl Jasmonate) on amaranthin synthesis was exploreded. MeJA ( 0.02

Key words:  Amaranthus mangostanus L., methyl jasmonate, wounding, amaranthin