生物学杂志

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绿潮暴发期间我国青岛漂浮铜藻的分子鉴定

  

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    1. 中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室, 烟台264003;
    2. 中国科学院大学, 北京 100049; 3. 曲阜师范大学生命科学学院,曲阜 273165; 4. 上海海洋大学水产与生命学院,上海 201306
  • 出版日期:2016-02-18 发布日期:2016-02-18
  • 通讯作者: 秦松,研究员,博士生导师,主要从事分子藻类学,E-mail: sqin@yic.ac.cn。
  • 作者简介:陈军,硕士,藻类分子生态学,E-mail: junchen@yic.ac.cn;王寅初,助理研究员,博士,主要从事藻类分子进化
  • 基金资助:
     
    中国科学院战略性先导科技专项(XDA1102040300)、国家自然科学基金项目(41176144, 41376139)和国家科技支撑计划(2013BAB01B01)资助

 
 Molecular phylogenetic analysis of floating Sargassum horneri associated with green tides in coastal area of Qingdao

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    ①Key Laboratory of Coastal Biology and Bioresource Utilization,Yantai Institute of Costal Zone Research, Chinese Academy of Sciences , Yantai 264003;
    ②University of Chinese Academy of Sciences , Beijing 100049
    ③College of Life Science, Qufu Normal University, Qufu 273165
    ④College of Fisheries and Life Science, Shanghai Ocean University,Shanghai 201306
    * Contact, E-mail: sqin@yic.ac.cn
  • Online:2016-02-18 Published:2016-02-18

摘要:  

2013年6月份,我国黄海连续第七年暴发绿潮灾害,同时出现了漂浮褐藻规模聚集现象,进一步增大了山东半岛绿潮防控工作的难度。为了对漂浮褐藻进行分子鉴定,本研究采用PCR扩增和序列测定的方法,获得了漂浮褐藻的5.8S rDNA-ITS序列。通过与已经报道的其他马尾藻属5.8S rDNA-ITS序列比对及系统发育分析,对绿潮暴发期间山东青岛近海出现的漂浮褐藻样品进行了分子鉴定。结果表明:该漂浮褐藻的5.8S rDNA-ITS基因序列全长1366 bp,其中ITS1序列长度为647 bp,5.8S rDNA序列长度为155 bp, ITS2序列长度为717 bp。序列比对分析显示,2013年绿潮暴发期间青岛海域的漂浮褐藻与我国浙江省和韩国江陵海区分布的铜藻样品遗传相似度高达99%。系统发育分析为马尾藻属铜藻(Sargassum horneri)。

关键词: 漂浮铜藻, 5.8S rDNA-ITS, 分子鉴定, 青岛

Abstract:  

In the Yellow Sea, large-scale green tides have broken out consecutively for eight years from 2007 to 2014. However, a small scale of floating brown algae appeared at the same time in coastal area of Shandong Peninsula, which gave more pressures including environmental impacts and economic losses. The 5.8S rDNA-ITS spacer regions of samples collected during the green tide were sequenced and analyzed. Multiple sequence alignment and phylogenetic analysis were performed to reveal the affinitybetween a Qingdao sample and four reported Sargassum samples. Our results indicated that the genetic identity of the algae from the green tide was of 99% similarity with Sargassum horneri from the coastal area of Zhejiang province and Jiangling, Korea. Phylogenetic analysis revealed that the floating sample clustered with Sargassum horneri closely, which proved the floating brown algae in Qingdao accompanied with green tides, was Sargassum horneri.