生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (4): 125-.doi: 10.3969/j.issn.2095-1736.2022.04.128

• 教学研究 • 上一篇    

吲哚乙酸对大蒜根尖细胞有丝分裂高峰期的影响

  

  1. 1. 阜阳师范大学 生物与食品工程学院， 阜阳 236037；
    2. 抗衰老中草药安徽省工程技术研究中心， 阜阳 236037
  • 出版日期:2022-08-18 发布日期:2022-08-15
  • 通讯作者: 屈长青，博士，教授，研究方向为天然产物研究与开发，E-mail: qucq518@163.com
  • 作者简介:陆娟，高级实验师，研究方向为微生物代谢，E-mail: 531818358@qq.com
  • 基金资助:
    安徽省线下课程（原精品线下开放课程）集体项目（2020kfkc379）

Effect of indoleacetic acid on themitosis peak in garlic apical cells

  1. 1. School of Biotechnology and Food Engineering, Fuyang Normal University, Fuyang 236037, China;
    2. Engineering Technology Research Center of Anti-aging Chinese Herbal Medicine, Fuyang 236037, China
  • Online:2022-08-18 Published:2022-08-15

PDF (PC)

31

摘要： 研究了水和10-9 mol/L吲哚乙酸培养的大蒜根尖在上午8点至下午4点间的有丝分裂情况。结果表明：改良的苯酚品红比龙胆紫和番红染色效果好；水培养的大蒜根尖存在最高峰期（下午2点）和次高峰期（上午10点），有丝分裂指数在1.91%～3.68%，细胞分裂的中、后期细胞所占比例在0.38%～1.47%；10-9 mol/L IAA培养的大蒜根尖有丝分裂仅存在高峰期（8：00），随后分裂指数几乎呈现为降低的趋势，有丝分裂指数在1.91%～9.11%，细胞分裂的中后期细胞所占比例在0.80%～3.55%。因此，在教学中为提高观察植物染色体实验成功率和实验效果，在大蒜根尖观察有丝分裂时，添加10-9 mol/L吲哚乙酸。

关键词: 大蒜, 有丝分裂, 高峰期, 吲哚乙酸

Abstract: The mitosis of garlic tips was studied from 8 a.m to 4 p.m, and the garlic was cultured in water and indoleacetic acid solution (10-9 mol/L). Results showed that the staining effect of garlic tips by modified phenol magenta dye solution was better than that by gentian violet and safranine. The mitosis peak and the second peak of garlic tips cultured in water were at 2 p.m and 10 a.m, the mitotic index was between 1.91% and 3.68%, and the proportion of the metaphase and anaphase cells was between 0.38% and 1.47%. But the peak of garlic tips mitosis cultured in indoleacetic acid solution was at 8 a.m, then the mitotic index showed almost downward trends, the mitotic index was between 1.91% and 9.11%, and the proportion of the metaphase and anaphase cells was between 0.80% and 3.55%. Therefore, in order to improve the mitosis experimental success rate and effect, it is recommended to culture garlic in indoleacetic acid solution (10-9 mol/L).

Key words: Allium sativum L., mitosis, peak, indoleacetic acid

中图分类号: