生物学杂志 ›› 2022, Vol. 39 ›› Issue (3): 72-.doi: 10.3969/j.issn.2095-1736.2022.03.072

• 研究报告 • 上一篇    下一篇

雷帕霉素对三角褐指藻岩藻黄素含量及关键酶基因表达的影响

  

  1. 1.宁波大学 海洋学院, 宁波 315832; 2.宁波大学 食品与药学学院, 宁波 315832
  • 出版日期:2022-06-18 发布日期:2022-06-17
  • 通讯作者: 龚一富,副教授,主要从事藻类物化学和分子生物学方面研究,E-mail: gongyifu@163.com
  • 作者简介:韦凤娟,硕士研究生,主要从事藻类生物化学和分子生物学方面研究,E-mail: 1124131754@qq.com
  • 基金资助:
    宁波市社发重大项目(2017C510002)

Effects of rapamycin on the content of fucoxanthin in Phaeodactylum tricornutum and the expression of key enzyme genes

  1. 1. School of Marine Sciences, Ningbo University, Ningbo 315832, China;
    2. College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China
  • Online:2022-06-18 Published:2022-06-17

摘要: 研究雷帕霉素(RPM)对三角褐指藻岩藻黄素含量的影响,探索岩藻黄素积累与光合作用反应之间的关系。结果表明,2.5~10 mmol/L RPM能显著促进藻细胞数及岩藻黄素含量积累,处理组岩藻黄素含量最高达5 mmol/L,含量提高40%。RT-qPCR结果表明,RPM处理24 h后藻细胞岩藻黄素合成基因pds表达水平与岩藻黄素含量变化相同,lcyb基因表达呈现下调趋势,光合作用相关基因psbA、rbcL、rbcS和fcpb表达水平均上调差异显著。主成分分析表明,岩藻黄素含量积累与光合作用相关基因rbcS、psbA和rbcL表达水平存在正相关性。RPM促进三角褐指藻岩藻黄素合成主要是通过光合作用相关基因rbcS、psbA和rbcL表达来实现,光合作用作为最关键因子影响三角褐指藻岩藻黄素积累。

关键词: 雷帕霉素, 三角褐指藻, 岩藻黄素, 光合作用

Abstract: The effects of rapamycin (RPM) on fucoxanthin content and the correlation between photosynthesis and fucoxanthin accumulation in Phaeodactylum tricornutum were studied in this paper. Results indicated that 2.5-10 mmol/L RPM promoted the growth of P. tricornutum cells and the accumulation of fucoxanthin content. When the RPM concentration was 5 mmol/L, the content of fucoxanthin was the highest, which increased 40%. RT-qPCR analysis results showed that 24 h after RPM treating, the expression level of pds gene was consistent with the change trend of fucoxanthin content in P. tricornutum. The expression of lcyb gene showed a downward-regulated trend compare with that of the treatment group. The expression levels of psbA, rbcL, rbcS and fcpb genes related to the photosynthetic were significantly up-regulated. Principal component analysis showed that the accumulation of fucoxanthin content was positively correlated with the expression levels of photosynthetic rbcS, psbA, and rbcL genes.This result provided a theoretical basis for further exploring the biosynthesis mechanism of fucoxanthin in P. tricornutum and the correlation between photosynthesis and fucoxanthin content.

Key words: rapamycin, Phaeodactylum tricornutum, fucoxanthin, photosynthesis

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