生物学杂志

生物学杂志 ›› 2020, Vol. 37 ›› Issue (4): 54-.doi: 10.3969/j.issn.2095-1736.2020.04.054

• 研究报告 • 上一篇    下一篇

甘露醇兼养三角褐指藻生产多不饱和脂肪酸和岩藻黄质

  

  1. 1. 青岛大学 环境科学与工程学院， 青岛 266071；2. 中国科学院 青岛生物能源与过程研究所, 青岛 266101
  • 出版日期:2020-08-18 发布日期:2020-08-10
  • 通讯作者: 张培玉，教授，研究方向为环境微生物，E-mail: envbio@163.com
  • 作者简介:丁晓婷，硕士研究生，研究方向为微藻生理生化，E-mail: 17806248403@163.com
  • 基金资助:
    国家自然科学基金（No. 31602154）；国家重点研究发展计划（2016YFB0601001）

Mixotrophic cultivation of Phaeodactylum tricornutum with mannitol to produce polyunsaturated fatty acids and fucoxanthin

  1. 1. College of Environmental Science and Engineering, Qingdao University, Qingdao 266071; 2. Qingdao Institute of Bioenergy and Bioprocess Technology， Chinese Academy of Sciences， Qingdao 266101， China
  • Online:2020-08-18 Published:2020-08-10

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摘要： 探讨在三角褐指藻（Phaeodactylum tricornutum）培养过程中，使用甘露醇作为有机碳源，对三角褐指藻的生长，以及多不饱和脂肪酸含量和岩藻黄质积累的影响进行分析。通过验证，选择2 g/L甘露醇为最佳添加浓度。同时添加0.2 g/L茶多酚可以有效促进三角褐指藻对甘露醇的利用。经过10 d兼养培养，同光合自养培养组相比，三角褐指藻最终生物量提高了63%，岩藻黄质产量达到75.3 mg/L，提高了90%，EPA（Eicosapentaenoic acid）占总油脂的含量达到36%，提高了35%。为今后三角褐指藻的兼养生产提供了一种有效的有机碳源和活性添加剂，为提高EPA及岩藻黄质产量提供了基础。

关键词: 三角褐指藻, 甘露醇, 茶多酚, EPA, 岩藻黄质

Abstract: In this study, mannitol was used as organic carbon source to investigate its effects on the accumulation of biomass, polyunsaturated fatty acids and fucoxanthin of Phaeodactylum tricornutum under this mixtrophic condition. The concentration of mannitol at 2 g/L was finally selected for the cultivation. Furthermore, tea polyphenols at 0.2 g/L could effectively promote the utilization of mannitol. Compared with that of autotrophic culture, the final biomass was increased by 63%, and the yield of fucoxanthin reached to 75.3 mg/L, increased by 90%; EPA（Eicosapentaenoic acid） accounted for 36% of the total lipid, increased by 35%. This study provided a potential mixtrophic carbon source and antioxidant for improving production of EPA and fucoxanthin by P. tricornutum.

Key words: Phaeodactylum tricornutum, mannitol, tea polyphenols, EPA, fucoxanthin

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