生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (6): 87-.doi: 10.3969/j.issn.2095-1736.2021.06.087

• 综述与专论 • 上一篇    下一篇

邻近标记技术在神经生物学中的应用与展望

  

  1. 1.河南科技大学 基础医学院，洛阳471000；2.河南省血栓与止血国际联合实验室，洛阳471000
  • 出版日期:2021-12-18 发布日期:2021-12-15
  • 通讯作者: 沈国民，博士，副教授，主要从事生物酶的结构与功能研究，E-mail:guominshen@haust.edu.cn
  • 作者简介:范玲玲，博士，主要从事神经退行性疾病的中枢机制研究，E-mail: emmafll@163.com
  • 基金资助:
    国家自然科学基金项目（No. 81770140）；河南省科技攻关项目（No. 182102410079）

Proximity labeling technique and its application and research prospect in neurobiology

  1. 1.School of Basic Medicine, Henan University of Science and Technology, Luoyang 471000， China； 2.Henan International Joint Laboratory of Thrombosis and Hemostasis, Luoyang 471000， China
  • Online:2021-12-18 Published:2021-12-15

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摘要： 通过综述基于过氧化物酶和生物素连接酶的邻近标记技术的原理、应用及常见问题，重点回顾目前邻近标记技术在神经生物学领域主要用于突触亚区分子成分的研究并取得一些成果，分析在应用过程中面临的活体脑组织中探针输入和高背景等挑战。展望该技术将为系统地鉴定神经组织中高度复杂的动态相互作用蛋白质组及亚细胞结构蛋白质组提供前所未有的机会，也为寻找神经生物学中的关键分子提供有效途径。

关键词: 临近标记, 生物素连接酶, 氧化物酶, 生物素, 神经生物学

Abstract: The principle, application and common problems of peroxidase-based PL and biotin ligase-based PL are introduced. This review focuses on the current application of PL technique in the study of synaptic subregion molecular components in the field of neurobiology and its achievements, and analyzes the challenges faced in the application process, such as probe input and high background in living brain tissue. It is pointed out that this technique will provide an unprecedented opportunity to systematically identify the highly complex proteome of dynamic interaction and subcellular structure in neural tissue, and also provide an effective approach to search for key molecules in neurobiology.

Key words: proximity labeling, biotin ligase, peroxidase, biotin, neurobiology

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