生物学杂志

生物学杂志

• 研究报告 • 上一篇    下一篇

3种冷季型草坪草对四氯乙烯胁迫的抗性响应

  

  1. 合肥学院 生物与环境工程系 安徽省环境污染防治与生态修复协同创新中心, 合肥 230601
  • 出版日期:2019-08-18 发布日期:2019-08-18
  • 通讯作者: 吴克, 教授, 硕士生导师, 主要从事环境工程和酶工程学研究, E-mail: wuke@hfuu.edu.cn
  • 作者简介:晋松, 硕士, 讲师, 主要从事环境生态学研究, E-mail: jinsong@hfuu.edu.cn
  • 基金资助:
    水体污染控制与治理科技重大专项(2017ZX07603-003); 安徽高校自然科学研究重大项目(KJ2016SD50); 安徽省高校自然科学研究一般项目(KJ2015B1105901); 合肥学院科研发展基金自然科学重大项目(16ZR05ZDA)

Resistance response of three cool-season turfgrass to tetrachloroethylene stress

  1. Collaborative Innovation Center for Environmental Pollution Control and Ecological Restoration of Anhui Province,Department of Biological and Environmental Engineering, Hefei University, Hefei 230601, China
  • Online:2019-08-18 Published:2019-08-18

PDF (PC)

130

摘要： 通过水培试验研究了梯度浓度四氯乙烯(PCE)对高羊茅、鸭茅与黑麦草的种子萌发、膜脂过氧化水平及抗氧化酶系统的影响。结果表明：梯度浓度的PCE处理对高羊茅种子萌发无显著影响，但对黑麦草、鸭茅种子萌发有抑制效应。随着PCE浓度升高(0~20 mg/L)，供试幼苗叶片细胞质膜受损，叶片电导率、MDA和脯氨酸含量持续上升，其中高羊茅叶片脯氨酸积累量最高。植株叶片中SOD、POD、CAT等3种抗氧化酶活性均先升后降，其中鸭茅、黑麦草的3种酶活性峰值均出现于PCE浓度8 mg/L时；而高羊茅幼苗叶片的CAT、SOD酶活则在PCE浓度为12 mg/L时达到峰值。以上结果表明，3种草坪草中，高羊茅对PCE污染的抗性能力最强、黑麦草最敏感；脯氨酸含量可作为衡量PCE胁迫下植物抗逆性的重要生理指标。

关键词: PCE胁迫, 冷季型草坪草, 抗氧化酶系统, 抗逆性

Abstract: The effect of tetrachloroethylene (PCE) on the seeds germination, the level of membrane lipid peroxidation and antioxidase system of Festuca elata Keng ex E.-Alexeev, Dactylis glomerata L. and Lolium perenne L. were studied by hydroponic experiment. The results revealed that the prolonged treatment with PCE (0-20 mg/L) produced no significant effect on the seed germination of F. elata, but the germination energy, germination rate and germination index of the L. perenne and D. glomerata were lasted inhibited. The plasma membrane of three turfgrasses’ blades suffered lasting stress, accompanied with enhanced conductivity, MDA content, and cell membrane permeability as well as increasing with highly concent of proline in blades with the increase of the concentration of PCE (0-20 mg/L), while there was the highest content of proline accumulated in the blades of F. elata. All of three grasses displayed decreasing antioxidase activity after initial increase. The peak value of the enzymatic activity of both L. perenne and D. glomerata appeared at 8 mg/L of the PCE concentration, whereas the F. elata reached its peak values in CAT and SOD activity when it was 12 mg/L. It could be inferred that among three kinds of turfgrass, F. elata had the strongest resistance, while L. perenne was most sensitive to PCE, and proline content could be used as the physiological index to the resistance under PCE stress.

Key words: PCE stress, cool-season turfgrass, antioxidant enzyme system, stress resistance

中图分类号: