生物学杂志

生物学杂志

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骨髓基质细胞中MGAT3催化的平分型N-糖链对血液细胞黏附和迁移的影响

  

  1. 1. 江南大学 生物工程学院， 无锡 214122； 2. 西北大学 生命科学院， 西安 710069
  • 出版日期:2018-10-18 发布日期:2018-10-18
  • 通讯作者: 李想，博士，教授，研究方向为骨髓微环境对肿瘤细胞影响，E-mail：xiangli@nwu.edu.cn
  • 作者简介:张鑫，硕士研究生，专业方向为制药工程，E-mail：zhangxin_thin@163.com
  • 基金资助:
    国家自然科学基金（NO.31400691）

The influence of MGAT3-catalyzed bisecting GlcNAc in stroma cells on cell adhesion and migration of hematopoietic cells

  1. 1. School of Biotechnology, Jiangnan University, Wuxi 214122;2. College of Life Sciences, Northwest University, Xi′an 710069, China
  • Online:2018-10-18 Published:2018-10-18

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摘要： 骨髓基质细胞是造血微环境的重要组成之一，在促进造血干细胞生成血细胞的各个环节中起着十分重要的作用。研究了人骨髓基质细胞HS5中抑制平分型N-糖链的表达，并观察其对共培养体系中血液细胞黏附和迁移的影响。利用基因工程技术在HS5中沉默平分型N-糖链合成中的关键酶，即N-乙酰氨基葡萄糖转移酶（MGAT3），用凝集素染色检测发现沉默MGAT3后HS5中平分型N-糖链的表达量降低。通过共培养发现，HS5中的平分型N-糖链降低后，血液细胞KG1a和PL-21对HS5的黏附能力降低,迁移能力也随之降低。由此表明，平分型N-糖链参与的骨髓造血微环境对调控血液细胞黏附和迁移方面有重要的生物学功能。

关键词: MGAT3；平分型N-糖链；基质细胞HS5；血液细胞, 细胞黏附；细胞迁移

Abstract: Bone marrow stromal cells are one of the important components of hematopoietic microenvironment, and play a very important role in regulating hematopoiesis. In this study, we inhibited the expression of bisected N-glycan in stroma cells and observed its effect on cell adhesion and migration of hematopoietic cells in co-culture system. N-acetylglucosaminyltransferase-III (so called MGAT3)， the key enzymes in the synthesis of N-glycan was silenced in human stroma cells HS5 using genetic engineering techniques. We found the level of bisecting GlcNAc significantly decreased in HS5 after MGAT3 was inhibited. Cell adhesion and migration of hematopoietic cells KG1a and PL-21 decreased after co-cultured with HS5 that inhibitg the expression of bisecting GlcNAc. Our data indicated that bisecting GlcNAc in stroma cells had important roles in regulating hematopoietic cells adhesion and migration.

Key words: MGAT3, bisecting GlcNAc, stromal cell, hematopoietic cell HS5, cell adhesion, cell migration