生物学杂志

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FASLG蛋白的序列分析及结构研究

  

  1. 西南交通大学生命科学与工程学院,成都610031
  • 出版日期:2016-02-18 发布日期:2016-02-18
  • 通讯作者: 李萍 ( 1962-),女,教授,博士,研究方向:生物化学与分子生物学,E- mail: wu_mengting@163. Com 徐柳(1970-),女,副教授,博士,研究方向:生物化学与分子生物学,E-mail:zxuliu@163.com
  • 作者简介:麦秀英(1989-),男,汉,硕士研究生,研究方向:生物化学与分子生物学,E-mail:
  • 基金资助:
    教育部留学回国人员科研启动基金(项目号2013S03008)

Sequence Analvsis and Structure Study of FASLG Protein

  1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031
  • Online:2016-02-18 Published:2016-02-18

摘要: 目的:分析FASLG的序列特征,结构特征及与受体FAS的结合位点,为后续研究作用机制提供理论基础和实验思路。方法:NCBI蛋白质数据库获取FASLG的序列信息,并使用生物信息学软件ProtParam,ProtScale,SignalP 4.1,TMHMM Server v. 2.0,ClustalX,SWISS-MODEL和AutoDock等进行序列分析。结果:FASLG序列全长为281个氨基酸,偏碱性,为不稳定的亲水性蛋白;为跨膜蛋白,不含信号肽;有一个FAS受体结合域。FASLG的三维结构包含74个α螺旋,20个B折叠, 56个延展片段,其余131个则全为无规则卷曲。FASLG蛋白通过15个氨基酸与其受体FAS蛋白相互耦合,从而形成FAS/FASLG信号通路来诱导凋亡。结论:得到FASLG的序列特征、结构及受体结合位点,对后续研究FAS/FASLG信号通路及miR-21和FASLG在结肠癌细胞中的作用提供新思路和方向。

关键词: FASLG, 生物信息学, 分子对接

Abstract:  

Objective: To provide theoretical basis and experimental ways for follow-up study, analysising the sequence signature, structure and receptor binding sites of FAS protein. Method: the sequence of FASLG protein was downloaded from NCBI database, which is studied by ProtParam,ProtScale,SignalP 4.1,TMHMM Server v. 2.0,ClustalX,SWISS-MODEL and AutoDock. Result: FASLG has 281 amine acids, which is alkaline, unstable hydrophilic and transmembrane protein. FASLG has no signal peptide and receptor binding cites of FAS. The 3D structure of FASLG consisted by 74 α helices,20 B sheets, 56 Extending segments and 131 coils. The FASLG protein couples with its receptor protein of FAS by 15amino acids,then they will form the FAS/FASLG signaling pathways to induce cell apoptosis. Conclusion: The sequence signature, 3D structure and receptor binding cites are obtained, which provides a new direction of studying the FAS/FASLG signaling pathway and knowing the role of miR-21 and FASLG in colon cancer cells.

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