生物学杂志

生物学杂志

• 技术方法 • 上一篇    下一篇

多花黄精组培体系建立及薯蓣皂苷等含量测定

  

  1. 1. 安徽大学 资源与环境工程学院， 合肥 230601； 2. 安徽大学 生命科学学院， 合肥 230601
  • 出版日期:2017-12-18 发布日期:2017-12-18
  • 通讯作者: 王 钰，教授，博士，研究方向为种植资源保护与利用，E-mail: 03136@ahu.edu.cn
  • 作者简介:刘芳源，硕士，研究方向为种植资源保护与利用，E-mail:liufy522@163.com
  • 基金资助:
    安徽大学研究生学术创新研究项目（YQH100245）

Establishment of tissue culture system and determination of yam saponins and diosgenin in Polygonatum sibiricum Hua

  1. 1. School of Resources and Environmental Engineering; 2. School of Life Sciences, Anhui University, Hefei 230601, China
  • Online:2017-12-18 Published:2017-12-18

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摘要：  为了加快繁殖多花黄精，建立多花黄精组培体系，对组培快繁培养基配方等进行研究，并利用UPLC法测定多花黄精中薯蓣皂苷和薯蓣皂苷元的含量。结果显示，最适快繁培养基为MS+1.0 mg/L 6-BA+0.5 mg/L NAA+0.3 mg/L IAA，炼苗最适基质为泥炭土+沙子(2∶ 1)，多花黄精中薯蓣皂苷和薯蓣皂苷元总含量范围为90.49~174.42 μg/g。

关键词: 多花黄精, 组织培养, 薯蓣皂苷, 薯蓣皂苷元, UPLC

Abstract: In order to accelerate the reproduction of Polygonatum sibiricum Hua, the paper established the tissue culture system of Polygonatum sibiricum Hua and studied the rapid propagation of tissue culture medium. The UPLC method was used for the determination of yam saponins and diosgenin in Polygonatum sibiricum Hua. The results showed that the optimal medium was MS+1.0 mg/L 6-BA+0.5 mg/L NAA+0.3 mg/L IAA. The optimal medium for hardening was peat soil and sand(2∶ 1). The total content of yam saponins and diosgenin was in the range of 90.49-174.42 μg/g.

Key words: Polygonatum sibiricum Hua, tissue culture, yam saponins, diosgenin, UPLC