生物学杂志

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基于磁性纳米探针的乙肝前S1抗原的快速磁性免疫层析方法的建立

  

  1.  
    1上海海洋大学食品学院上海水产品加工及贮藏工程技术研究中心,上海,201306;
    2. 南京爱纳玛斯生物医药科技有限公司,江苏南京,211100;
    3. 同济大学医学院 免疫与炎症研究中心,上海,200092;
    4. 同济大学医学院 生物医学工程与纳米科学研究院,上海,200092
  • 出版日期:2016-02-18 发布日期:2016-02-18

 
Establishment of HBV rapid magnetic immunochromatographicmethod based on magnetic nanoprobe for Pre-S1 antigen

  1. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306; Nanjing iNanoMax Medtech Limited Company211100; Immune and inflammatory research center , Tongji University School of Medicine, Shanghai, 200092; The Institute for Biomedical Engineering and Nano Science, Tongji University School of Medicine, Shanghai, 200092, Nanjing, jiangsu province,
  • Online:2016-02-18 Published:2016-02-18
  • Contact: 卢瑛,副教授,硕士生导师,主要从事纳米生物技术、食品质量与安全控制研究,E-mail: y-lu@shou.edu.cn;

摘要:  

为了建立快速灵敏的乙肝磁性免疫层析检测方法,本文选用前S1抗原作为乙肝检测标志物,将前S1抗原的特异性单抗偶联到磁珠表面,制备了乙肝的特异性磁性纳米探针,并以此探针作为检测标记物制备了乙肝的磁性试纸条。采用乙肝临床血样,对所构建磁性试纸条的检测灵敏度、稳定性和重现性等检测性能进行了评价,并和市售的乙肝ELISA试剂盒进行了对比分析。与ELISA试剂盒的对比结果显示,本研究所建立的乙肝磁性试纸条灵敏度较ELISA法2倍,具有较好的稳定性(25 ℃可保存半年)和重现性;这两种方法对441份临床血样的检测结果一致性高达97%。本研究可为今后乙肝的床边早期诊断产品开发提供技术支撑。

关键词: 前S1抗原, 纳米磁珠, 乙肝, 免疫层析

Abstract:  

 To establish a rapidmethod for HBV in serum samples of people, in this study, PreS1 antigen is selected as markers for HBV detection. Specific antibody against PreS1 was coupled onto the surface of magnetic beads to prepare magnetic nanoprobe. HBV magnetic test strip labeled by magnetic nanoprobe was constructed. HBV clinical serum samples were used as detection samples. The detection performance of magnetic test strip including sensitivity, stability and repeatability was performed. The developed magnetic test strip was compared with commercially ELISA kit of HBV. Comparison results showed that sensitivity of our test strip was 2 folds higher than ELISA method. Good stability (can save half a year in 25 ) and reproducibility was assessed for the magnetic test strip. The consistency of these two methods was as high as 97% (n=441). Our study can provide technological support of point of care products for early diagnosis of HBV. and sensitive detection

Key words:  PreS1 antigen, nano magnetic beads, HBV, immunochromatographic