生物学杂志

生物学杂志

• 技术方法 • 上一篇    下一篇

控制哈茨木霉菌体形态提高β-1,3-葡聚糖内切酶活的研究

  

  1. 1. 江南大学 生物工程学院 糖化学与生物技术教育部重点实验室，无锡 214122;2. 江苏瑞光生物科技有限公司，无锡 214122
  • 出版日期:2016-12-18 发布日期:2016-12-18
  • 通讯作者: 郑志永，副教授，主要研究方向为微生物生理学和生物反应过程控制， E-mail: zhiyong@jiangnan.edu.cn
  • 作者简介:王剑，硕士，主要研究方向为发酵工学，E-mail:wangjian597@163.com
  • 基金资助:
    国家自然科学基金项目（31171640, 31271888）；无锡市科技支撑计划（农业, CLE01N1208）；无锡市130计划

Enhanced endo-β-1,3-glucanase produciton through morphology control of Trichoderma harzianum

  1. 1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University,Wuxi 214122; 2. Jiangsu Rayguang Biotech Co. Ltd., Wuxi 214122, China
  • Online:2016-12-18 Published:2016-12-18

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摘要： 哈茨木霉的深层培养菌体形态与β-1,3-葡聚糖内切酶酶活之间有密切关系。通过单因素试验优化了控制哈茨木霉菌球形态的培养条件为：摇瓶装液量100 mL/500 mL，接种量5%(V/V)，初始pH 6.5，回旋式摇床转速180 r/min，在培养基中添加0.3%(m/V)的吐温-80或0.3%(m/V)的CMC-Na。在该条件下，菌球直径控制在(0.42±0.09)mm左右，β-1,3-葡聚糖内切酶酶活达到(111.9±7.5)U/mL。进一步通过正交优化，β-1,3-葡聚糖内切酶酶活提高到了135.2±8.3 U/mL，同时得出各种因素对菌球直径的影响顺序为：接种量>CMC-Na>吐温-80。

关键词: 菌体形态；&beta, -1,3-葡聚糖内切酶；哈茨木霉

Abstract: There is a close relationship between morphology of Trichoderma harzianum and endo-β-1,3-glucanase production. The culture conditions for good morphology of Trichoderma harzianumin was optimized by single factor experiment, and the result showed as follow: 100 mL medium in 500 mL Erlenmeyer flask, 5% (V/V) of inoculum volume. The initial pH of the medium was 6.5 and shaking speed was 180 r/min, 0.3%(m/V) Tween-80 or 0.3%(m/V) CMC-Na was added into the medium as dispersing agents. The mycelia pellet diameter was about (0.42±0.09) mm and the activity of endo-β-1,3-glucan was (111.9±7.5) U/mL. In the further orthogonal optimization, the activity of endo-β-1,3-glucan reached (135.2±8.3) U/mL, and the influence order of culture conditions to mycelia pellet diameter was inoculum volume >CMC-Na> Tween-80.

Key words: morphology, endo-β-1,3-glucanase, Trichoderma harzianum