生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (5): 74-.doi: 10.3969/j.issn.2095-1736.2026.05.074

• 技术方法 • 上一篇    下一篇

菠萝蛋白酶类过氧化物酶活性检测葡萄糖研究

李全发1， 滕祎梦1， 周荣杰1， 刘亚楠1， 王宝娟1,2   

  1. 1. 安徽师范大学 生命科学学院， 芜湖 241000；
    2. 分子酶学与重大代谢性疾病机理研究安徽省重点实验室， 芜湖 241000
  • 出版日期:2026-10-18 发布日期:2026-10-20
  • 通讯作者: 王宝娟，博士，副教授，研究方向为酶学、细胞分子生物学及生物纳米材料，E-mail：wangbaojuan@ahnu.edu.cn
  • 作者简介:李全发，硕士，高级实验师，研究方向为酶学、新型酶活性的探索及相关生物检测技术的开发，E-mail：liquanfa@ahnu.edu.cn
  • 基金资助:
    国家自然科学基金项目（22376002）； 安徽省自然科学基金项目（2108085MC78）； 大学生创新创业平台训练项目（X202410370049）

Study of glucose detection with peroxidase-like activity of bromelin

LI Quanfa1, TENG Yimeng1, ZHOU Rongjie1, LIU Ya’nan1, WANG Baojuan1,2   

  1. 1. School of Life Sciences, Anhui Normal University, Wuhu 241000, China; 2. Anhui Provincial Key
    Laboratory of Molecular Enzymology and Mechanisms of Major Metabolic Diseases, Wuhu 241000, China
  • Online:2026-10-18 Published:2026-10-20

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摘要： 本研究初次证实菠萝蛋白酶具有类过氧化物酶活性，并通过酶学性能鉴定证实其过氧化物酶活性的最适反应温度为40 ℃，最适反应pH为5.0，且以H2O2或TMB为底物时，其比酶活分别为16.58 U/mg、18.41 U/mg。进一步将菠萝蛋白酶与葡萄糖氧化酶偶联，构建了一种级联催化体系，实现了对葡萄糖的宽范围和灵敏性检测，该体系检测范围为5~400 μmol/L，检测限为1.5 μmol/L。此外，该体系对葡萄糖检测具有良好的选择性和抗干扰性，可有效应用于人类血液和尿液样本中葡萄糖的检测。因此，该研究不仅为糖尿病诊断提供了一种高效、灵敏、操作简便且成本效益高的新型可视化检测方法，还成功拓宽了菠萝蛋白酶在酶学领域的应用范围，为酶学研究和应用注入新活力。

关键词: 级联催化, 灵敏检测, 选择性, 抗干扰性

Abstract: his study demonstrated for the first time that bromelin exhibited peroxidase-like activity. Enzymatic characterization confirmed that the optimal temperature and pH for its peroxidase-like activity were respectively 40 ℃ and 5.0, and with H2O2and TMB as substrates, the specific enzyme activities were 16.58 U/mg and 18.41 U/mg, respectively. Furthermore, a cascade catalytic system was constructed by coupling bromelin with glucose oxidase, enabling broad-range and highly sensitive glucose detection with a linear range of 5－400 μmol/L and a detection limit of 1.5 μmol/L. Additionally, the system demonstrated excellent selectivity and resistance to interference for glucose detection, enabling its effective application in the analysis of human blood and urine samples. Therefore, this research not only provided an efficient, sensitive, and visual detection method for diabetes diagnosis, characterized by operational simplicity and excellent cost-effectiveness, but also successfully expanded the application scope of bromelain in enzymology, bringing new vitality into enzymatic research and applications.

Key words: cascade catalysis, sensitive detection, selectivity, anti-interference ability

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