生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (5): 103-.doi: 10.3969/j.issn.2095-1736.2025.05.103

• 综述与专论 • 上一篇    下一篇

链霉菌聚酮类化合物和非核糖体多肽的生物合成及菌株育种研究进展

邹　超1，2， 孙　博2， 宋　佳2， 刘宽博3， 张万忠1， 赵　晨2   

  1. 1. 沈阳化工大学 化学工程学院， 沈阳 110142； 2. 国家粮食和物资储备局科学研究院 中心实验室，
    北京 100037； 3. 国家粮食和物资储备局科学研究院 粮食品质营养研究所， 北京 100037
  • 出版日期:2025-10-18 发布日期:2025-10-14
  • 通讯作者: 张万忠，教授，研究方向为生物分离工程、精细化学品的合成和生物无机化学，E-mail：lzwz2004@sina.com；赵晨，副研究员，研究方向为粮油资源微生物活性天然产物开发，E-mail： zc@ags.ac.cn；张万忠和赵晨为共同通信作者
  • 作者简介:邹超，硕士研究生，研究方向为药学，E-mail：m13591947366@163.com
  • 基金资助:
    中央级公益性基本科研业务费专项(ZX2421，ZX2453)

Research progress on the biosynthesis of polyketide compounds and non-ribosomal peptides in Streptomyces and the breeding of strains

ZOU Chao1,2, SUN Bo2, SONG Jia2, LIU Kuanbo3, ZHANG Wanzhong1, ZHAO Chen2   

  1. 1. College of Chemical Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China;
    2. Central Laboratory, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China;
    3. Institute of Grain Quality and Nutrition Research, Academy of National Food and Strategic Reserves
    Administration, Beijing 100037, China
  • Online:2025-10-18 Published:2025-10-14

摘要： 链霉菌（Streptomyces）作为一种重要的微生物类群，能够产生多种天然产物，包括抗癌、抗病毒、抗真菌和抗寄生虫等活性物质，在医药、农业及化工等领域发挥着重要作用。链霉菌的次级代谢产物主要包括聚酮类化合物和非核糖体多肽。由于链霉菌的代谢网络和调控机制极为复杂，加之其生物合成基因簇在大多数环境下都处于沉默状态，导致天然产物的产量较低，限制了其工业化生产的应用。因此，研究如何通过激活沉默的生物合成基因簇以及采用分子生物学育种策略来提高天然产物的产量具有重要意义。针对在农业方面具有重要应用价值的次级代谢产物，主要介绍链霉菌聚酮类化合物和非核糖体多肽的生物合成途径，综述诱变选育、碳、氮、磷代谢通路改造及异源表达等近年来提高链霉菌天然产物产量的方法，并展望结合高通量筛选及计算机预测的研究方向。以期为提高链霉菌次级代谢产物的研究提供参考，为新型聚酮类化合物和非核糖体多肽的开发与应用提供基础。

关键词: 链霉菌, PKS系统, NRPS系统, 异源表达, 碳、氮、磷代谢通路改造, 诱变育种

Abstract: Streptomyces, as an important group of microorganisms, produces a wide range of natural products, including anticancer, antiviral, antifungal, and antiparasitic bioactive compounds, and plays a crucial role in pharmaceuticals, agriculture, and the chemical industry. The secondary metabolites ofStreptomycesmainly consist of polyketide compounds and non-ribosomal peptides. However, due to the extremely complex metabolic network and regulatory mechanisms ofStreptomyces, coupled with the fact that their biosynthetic gene clusters are silent under most environmental conditions, the yield of natural products is low, which limits their application in industrial production. Therefore, studying how to activate silent biosynthetic gene clusters and using molecular biology breeding strategies to increase the production of natural products are of great significance. Given the agricultural significance of secondary metabolites, this review outlined the biosynthetic pathways of polyketide compounds and non-ribosomal peptides inStreptomyces, and summarized the strategies to improve the yield of natural products inStreptomycesin recent years, such as mutation breeding, carbon, nitrogen and phosphorus metabolic pathway modification and heterologous expression. Future research directions integrating high-throughput screening with computational prediction were proposed, aiming to advance the research of secondary metabolites ofStreptomycesand provide a basis for the development and application of polyketide compounds and non-ribosomal peptides.

Key words: Streptomyces, PKS system, NRPS system, heterologous expression, carbon, nitrogen, phosphorus metabolic pathway engineering, mutagenesis breeding

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