生物学杂志

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云南热区拟盘多毛孢抑菌活性筛选

  

  1. 西南林业大学生命科学学院,云南 昆明 650224
  • 出版日期:2015-12-18 发布日期:2015-12-18
  • 通讯作者: 李 靖,硕士,副教授,主要从事植物病害生物防治研究,E-mail:lijing@swfu.edu.cn.
  • 作者简介:李向楠,在读硕士研究生,生化与分子生物学专业
  • 基金资助:
    国家自然科学基金(31260177)

Activity screening of Pestalotiopsis sp. from tropical area in Yunnan province on plant pathogens

  1. Life Science College, Southwest Forestry University, Kunming, 650224 , China
  • Online:2015-12-18 Published:2015-12-18
  • Contact: 李 靖,硕士,副教授,主要从事植物病害生物防治研究,E-mail:lijing@swfu.edu.cn.

摘要: 本实验对分离自云南热区并鉴定到种的10株拟盘多毛孢进行了抗植物病原菌活性筛选。采用滤纸片法研究了10株拟盘多毛孢对10种常见植物病原真菌的抑菌活性,获得可抑制番茄灰霉病原菌的拟盘多毛孢2株,抑制黄瓜枯萎病原菌的拟盘多毛孢2株,抑制三七丝核病原菌的拟盘多毛孢9株,抑制棉花枯萎病原菌的拟盘多毛孢6株,抑制白菜黑斑病原菌的拟盘多毛孢1株,抑制稻瘟病病原菌的拟盘多毛孢9株,抑制辣椒炭疽病原菌的拟盘多毛孢8株,抑制梨黑星病原菌的拟盘多毛孢5株,抑制辣椒镰刀病原菌的拟盘多毛孢8株,抑制小麦雪腐病原菌的拟盘多毛孢1株。其中侧柏拟盘多毛孢、芦荟拟盘多毛孢具有广谱抗菌活性。该研究结果为拟盘多毛孢菌株的利用及活性物质的分离奠定了基础。

关键词: 拟盘多毛孢, 抑菌活性, 滤纸片法

Abstract: Antimicrobial activities of 10 strains of Pestalotiopsis sp. on 10 strains of plant pathogens were studied through the method of filter paper. Results showed that two Pestalotiopsis sp. could inhibit Botrytis cinerea, two Pestalotiopsis sp. could inhibit Fusarium oxysporium sp. Cucumebrium, nine Pestalotiopsis sp. could inhibit Verticillium cinnabarium, six Pestalotiopsis sp. could inhibit Fusarium oxysporium sp. Vasinfectum, one Pestalotiopsis sp. could inhibit Alternaria brassicae, nine Pestalotiopsis sp. could inhibit Magnaporthe grisea, eight Pestalotiopsis sp. could inhibit Sclerotinia sclerotiorum, five Pestalotiopsis sp. could inhibit Venturia pyrina, eight Pestalotiopsis sp. could inhibit Fusarium solani, one Pestalotiopsis sp. could inhibit Typhula incarnate. Pestalotiopsis thujae, and Pestalotiopsis aloes showed broad-spectrum resistance. Results of this study laid a foundation for the further utilization of Pestalotiopsis sp. and separation of active substance.

Key words: Pestalotiopsis sp., antimicrobial activity, filter paper