生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (6): 35-.doi: 10.3969/j.issn.2095-1736.2025.06.035

• 研究报告 • 上一篇    下一篇

沙棘果抑菌活性成分研究

许仙明1,2， 樊金昊1,2， 刘超智2， 周俊英3， 王　军4， 孙桂鸿5， 郭明雄1,2   

  1. 1. 西藏大学 生态环境学院 青藏高原生物多样性与生态环境保护教育部重点实验室， 拉萨 850000；
    2. 武汉大学 生命科学学院 细胞稳态湖北省重点实验室， 武汉 430072； 3. 武汉大学中南医院检验科，
    武汉 4030071； 4. 湖北工业大学 生命科学与健康工程学院， 武汉 430068； 5. 武汉大学 泰康医学院
    （基础医学院） 过敏及免疫相关疾病湖北省重点实验室， 武汉 430071
  • 出版日期:2025-12-18 发布日期:2025-12-19
  • 通讯作者: 郭明雄，教授，研究方向为青藏高原特色藏药材和微生物资源调查及其保护利用，E-mail:guomx@whu.edu.cn
  • 作者简介:许仙明，硕士研究生，研究方向为青藏高原特色藏药材和微生物资源调查及其保护利用，E-mail:2638762933@qq.com
  • 基金资助:
    西藏自治区中央引导地方科技发展资金项目（XZ202301YD0040C）； 国家自然科学基金地区科学基金项目（32260173）

Study on anti-bacterial active components in sea buckthorn fruit

XU Xianming1,2, FAN Jinhao1,2, LIU Chaozhi2, ZHOU Junying3,WANG Jun4, SUN Guihong5, GUO Mingxiong1,2   

  1. 1. Key Laboratory of Biodiversity and Environment on the Qinghai-Xizang Plateau, Ministry of Education, School of
    Ecology and Environment, Xizang University, Lhasa 850000, China; 2. Hubei Key Laboratory of Cell Homeostasis,
    College of Life Sciences, Wuhan University, Wuhan 430072, China; 3. Department of Laboratory Medicine, Zhongnan
    Hospital of Wuhan University, Wuhan 430071, China; 4. School of Life and Health Sciences, Hubei University of
    Technology, Wuhan 430068, China; 5. Hubei Provincial Key Laboratory of Allergy and Immunology, Taikang
    Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan 430071, China
  • Online:2025-12-18 Published:2025-12-19

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摘要： 采用超高效液相色谱-四极杆-静电场轨道阱联用质谱（UPLC-Q-Exactive Orbitrap-MS/MS）技术，从沙棘果中鉴定出68种化学成分。利用网络药理学分析和分子对接技术，预测柯里拉京、表没食子儿茶素与没食子儿茶素等8种化合物为沙棘果中的潜在抑菌成分，它们可能与TNF、IL6与AKT1等10个靶点有良好的结合活性，并可能通过参与调节磷脂酰肌醇3激酶-蛋白激酶B（PI3K-Akt）、白细胞介素-17（IL-17）等信号通路，发挥抑菌功能。进一步通过体外最低抑菌浓度试验（MIC）证实柯里拉京、表没食子儿茶素、没食子儿茶素以及二氢槲皮素等4种化合物的抑菌活性，其中，柯里拉京与表没食子儿茶素联用时对金黄色葡萄球菌表现出协同抑菌作用，联用时MIC分别降至31.25 μg/mL与62.5 μg/mL。研究通过高分辨质谱技术、结合多个数据库数据，较为全面地揭示了沙棘果的化学成分谱，为阐释沙棘果的相关药理特性和传统药用功效奠定化学物质基础；通过整合网络药理学、分子对接技术与体外实验等多种方法，首次发现沙棘果中多种抑菌化学成分及其潜在作用机制，为沙棘果在抑菌方面的应用提供一定的理论依据。

关键词: 沙棘果, UPLC-Q-Exactive Orbitrap-MS/MS, 网络药理学, 分子对接, 抑菌活性成分

Abstract: Utilizing ultra-high performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q-Exactive Orbitrap-MS/MS), a total of 68 chemical constituents were identified from sea buckthorn fruit. Network pharmacology analysis and molecular docking predicted 8 potential antibacterial compounds in sea buckthorn fruit, including corilagin, epigallocatechin, and gallocatechin. These compounds might exhibit favorable binding affinities with key targets including TNF, IL6, and AKT1, potentially exerting antibacterial effects via the modulation of signaling pathways including PI3K-Akt and IL-17. Furthermore,in vitrominimum inhibitory concentration (MIC) assays confirmed the antibacterial efficacy of corilagin, epigallocatechin, gallocatechin, and taxifolin. Notably, the combination of corilagin and epigallocatechin demonstrated a synergistic inhibition againstStaphylococcus aureus, reducing MICs to 31.25 μg/mL and 62.5 μg/mL, respectively. This research, leveraging high-resolution mass spectrometry technology and integrating data from multiple databases, comprehensively characterized the chemical profile of sea buckthorn fruit, establishing a solid foundation for understanding its pharmacological properties and traditional medicinal efficacy. By integrated network pharmacology, molecular docking techniques, andin vitroexperiments, this study represented the first discovery of multiple antibacterial chemical components in sea buckthorn fruit, revealing their potential mechanisms, providing a theoretical basis for the application of sea buckthorn fruit in antibacterial and anti-infective therapies.

Key words: sea buckthorn fruit, UPLC-Q-Exactive Orbitrap-MS/MS, network pharmacology analysis, molecular docking, antibacterial active components

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