生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (4): 83-.doi: 10.3969/j.issn.2095-1736.2023.04.083

• 研究报告 • 上一篇    下一篇

不同消毒剂对藜麦种子表面消毒效果和萌发影响综合评价

朱丽丽1,2, 张业猛1,2, 李万才5, 赵亚利6, 陈志国1,3,4   

  1. 1. 中国科学院西北高原生物研究所, 西宁 810008; 2. 中国科学院大学, 北京 100049; 3. 中国科学院
    高原生物适应与进化重点实验室, 西宁 810008; 4. 青海省作物分子育种重点实验室, 西宁 810008;
    5. 互助县种子站, 海东 810500; 6. 乐都区种子站, 海东 810700
  • 出版日期:2023-08-18 发布日期:2023-08-10
  • 通讯作者: 陈志国，研究员，博士生导师，主要从事作物遗传育种研究，E-mail:zgchen@nwipb.cas.cn
  • 作者简介:朱丽丽，硕士研究生，主要从事作物遗传育种研究，E-mail:2636143756@qq.com
  • 基金资助:
    青海省重点研发与转化计划项目（2020-NK-122）； 中国科学院种子创新研究院项目（INASEED）

Comprehensive evaluation of the effect of different disinfectants on the surface disinfection of quinoa seeds and their germination

ZHU Lili1,2, ZHANG Yemeng1,2, LI Wancai5, ZHAO Yali6, CHEN Zhiguo1,3,4   

  1. 1. Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810008, China; 2. Chinese
    Academy of Sciences University, Beijing 100049, China; 3. Key Laboratory of Plateau Biological Adaptation and
    Evolution, Chinese Academy of Sciences, Xining 810008, China; 4. Key Laboratory of Crop Molecular Breeding of
    Qinghai Province, Xining 810008, China; 5. Huzhu County Seed Station, Haidong 810500, China; 6. Ledu District
    Seed Station, Haidong 810700, China
  • Online:2023-08-18 Published:2023-08-10

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摘要： 为明确种子表面消毒剂对藜麦的效应，以青藜11号和青藜12号藜麦种子为实验材料，选择5种常用消毒剂（KMnO4、H2O2、NaClO、HgCl2和75%乙醇），配制不同浓度（0.1%、0.5%、1%、1.5%、2%、2.5%和3%）和设置不同消毒时间（1、5、10、15、20、25和30 min），采用L49（41×72）正交试验，以种子萌发、幼苗生长、胚破裂率、异常发芽率和发霉率等为考察指标，综合分析不同消毒剂处理方法对藜麦种子的消毒效果和对种子萌发伤害。结果表明：消毒剂种类对藜麦种子消毒效果的差异较为明显，其中KMnO4和NaClO的消毒效果较好，HgCl2处理的藜麦种子污染率最小，但其对种子和幼苗的毒害作用较大，导致多数种子破裂和不正常发芽，不宜使用；H2O2处理对种子破坏较小，但消毒不彻底，污染率较高，达不到较为理想的消毒效果。依据隶属函数法筛选结果得出2.5% KMnO4处理15 min为藜麦种子的最佳消毒方法。

关键词: 藜麦种子, 消毒, 萌发, 主成分分析, 隶属函数法

Abstract: To clarify the effect of seed surface disinfectants on quinoa, using Qingli No.11 and Qingli No.12 quinoa grains as experimental materials, five commonly used disinfectants (KMnO4, H2O2, NaClO , HgCl2and 75% alcohol) were selected to prepare different concentrations (0.1%, 0.5%, 1%, 1.5%, 2 %, 2.5% and 3%) and set different disinfection time (1, 5, 10, 15, 20, 25 and 30 min). L49 (41×72) orthogonal experiment was used to investigate the seed germination, seedling growth, embryo rupture rate, abnormal germination rate and moldy rate. The disinfection effect of different disinfectants treatment methods on quinoa seeds was comprehensively analyzed. The results showed that the types of disinfectants had obvious effects on the disinfection effect of quinoa seeds. Among them, KMnO4and NaClO had better disinfection effects. The quinoa seeds treated with HgCl2had the least pollution rate, but it had a greater toxic effect on seeds and seedlings, causing most seeds to break and germinate abnormally, and should not be used. H2O2treatment has less damage to the seeds, but the pollution rate is high, and the ideal disinfection effect cannot be achieved. According to the screening results of membership function method, it was concluded that 2.5% KMnO4treatment for 15 min was the best disinfection method for quinoa seeds.

Key words: quinoa seed, disinfection, germination, principal component analysis, membership function method

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