生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (1): 69-.doi: 10.3969/j.issn.2095-1736.2023.01.069

• 研究报告 • 上一篇    下一篇

基于COI和Cytb基因序列的太湖河蚬遗传多样性分析

李大命1, 刘　洋1,2, 刘燕山1, 唐晟凯1, 谷先坤1, 殷稼雯1, 蒋琦辰1, 张彤晴1   

  1. 1. 江苏省淡水水产研究所 江苏省内陆水域渔业资源重点实验室, 南京 210017；
    2. 南京师范大学 海洋科学与工程学院， 南京 210023
  • 出版日期:2023-02-18 发布日期:2023-02-21
  • 作者简介:李大命, 博士, 研究员, 从事渔业资源和环境监测及评估研究, E-mail: ldm8212@126.com
  • 基金资助:
    江苏省自然科学基金面上项目(BK20191488)； 江苏省水生生物资源重大专项(ZYHB16-3)； 2019年淡水渔业资源监测项目(2019-SJ-018-2)

Genetic diversity analysis of Corbicula fluminea in the Tai Lake based on mitochondrial COI and Cytb gene sequences

LI Daming1, LIU Yang1,2, LIU Yanshan1, TANG Shengkai1, GU Xiankun1, YIN Jiawen1,JIANG Qicheng1, ZHANG Tongqing1   

  1. 1. Freshwater Fisheries Research Institute of Jiangsu Province, Key Laboratory of Fisheries Resources and
    Environment in Inland Water, Nanjing 210017, China;
    2. College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China
  • Online:2023-02-18 Published:2023-02-21

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摘要： 以线粒体COI和Cytb基因部分序列作为分子标记，对太湖河蚬(Corbicula fluminea)群体的遗传多样性进行分析。结果显示：COI和Cytb序列片段长度分别为614 bp和621 bp，两基因片段的碱基AT含量明显大于GC含量，碱基组成具有偏好性。COI序列有19个变异位点，定义14种单倍型，平均单倍型和核苷酸多样性分别为0.851±0.04和0.00745±0.00081，平均核苷酸差异数为4.572，单倍型之间的遗传距离为0.002至0.020；Cytb序列有19个变异位点，定义17种单倍型，平均单倍型和核苷酸多样性分别为0.896±0.028和0.00510±0.00051，平均核苷酸差异数为3.167，单倍型之间的遗传距离为0.002至0.013，河蚬的遗传多样性具有高单倍性多样性和高核苷酸多样性特征。中性检验结果和歧点分布图分析表明，太湖河蚬种群进化过程中保持相对稳定，未经历显著的种群扩张过程。

关键词: 河蚬, 遗传多样性, Cytb基因, COI基因, 种群历史

Abstract: The mitochondrial COI and Cytb partial gene sequences were used as molecular markers to analyze and evaluate the genetic diversity of Corbicula fluminea in the Tai Lake. The 614 bp-COI and 621 bp-Cytb gene sequences were obtained from 40 individuals of C. fluminea by PCR amplification and sequencing. Meanwhile, the content of A+T was much higher than that of G+C in both COI and Cytb sequences indicated that there was obvious bias for base composition. 19 variable sites were detected and 14 haplotypes were defined in COI sequences, the average haplotype diversity and nucleotide diversity were 0.851±0.04 and 0.00745±0.00081, respectively, the average number of nucleotide differences was 4.572, and the genetic distance among haplotypes ranged from 0.002 to 0.020. 19 variable sites were detected and 17 haplotypes were defined in Cytb sequences, the average haplotype diversity and nucleotide diversity were 0.896±0.028 and 0.00510±0.00051, respectively, the average number of nucleotide differences was 3.167, and the genetic distance among haplotypes ranged from 0.002 to 0.013. Therefore, the genetic diversity of C. fluminea showed high haplotype diversity and high nucleotide diversity pattern. The Tajima’s D and Fu’s Fs values of C. fluminea were negative but no significantly different, and mismatch distribution analysis showed a multiple type, indicating that C. flumineain the Tai Lake had remained relatively stable and had not experienced population expansion in the history. The present results provided scientific information for conservation and sustainable utilization of C. fluminea germplasm resources in Tai Lake.

Key words: Corbicula fluminea, genetic diversity, COI gene, Cytb gene, population history

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