生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (4): 28-.doi: 10.3969/j.issn. 2095-1736.2022.04.028

• 研究报告 • 上一篇    下一篇

DNA甲基化转移酶hsdM基因对维氏气单胞菌运动性的影响

  

  1. 海南大学 生命科学与药学院 热带生物资源教育部重点实验室，海口 570228
  • 出版日期:2022-08-18 发布日期:2022-08-15
  • 通讯作者: 马香，博士，副教授，研究方向为病原微生物致病性机制，E-mail: 993034@hainanu.edu.cn
  • 作者简介:陈婷，硕士研究生，研究方向为分子遗传学，E-mail: chenting9611@126.com
  • 基金资助:
    国家自然科学基金地区基金项目（31960027）; 国家自然科学基金青年基金项目（32000089）

DNA methyltransferase gene hsdM influences bacterial motility in Aeromonas veronii

  1. Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Life and Pharmaceutical Sciences,
    Hainan University, Haikou 570228, China
  • Online:2022-08-18 Published:2022-08-15

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摘要： Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Life and Pharmaceutical Sciences,
Hainan University, Haikou 570228, China

关键词: DNA甲基化, 甲基转移酶, 维氏气单胞菌, 细菌运动性

Abstract: To investigate the effects of methyltransferase gene hsdM of type I RM system on the motility of Aeromonas veronii, hsdM deletion strains ΔhsdM was constructed to explore the influences on the growth, motility and flagellum gene expressions of Aeromonas veronii. Growth curves showed that the growth rate of Δ hsdM strains began to decrease slowly in the late growth stage of logarithmic phase, while the overall growth levels of wild type and ΔhsdM mutant are similar; motility assay suggested that the deletion of hsdM significantly reduced the motility of the bacteria. RT-qPCR indicated that the expressions of some flagellum genes were significantly down-regulated in ΔhsdM strains. This study preliminarily explored the basic phenotypes of hsdM deletion related with bacterial motility, providing a theoretical foundation for further study on the mechanism of 6mA methylation on regulations of gene expressions.

Key words: DNA methylation, methyltransferase, Aeromonas veronii, bacterial motility

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