生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (5): 72-.doi: 10.3969/j.issn.2095-1736.2021.05.072

• 研究报告 • 上一篇    下一篇

以蝗虫翅为模板的仿生高分子膜血液相容性研究

  

  1. 1. 三明学院 资源与化工学院 药用植物开发利用福建省高校工程研究中心 福建省资源环境监测与
    可持续经营利用重点实验室， 三明 365004；2. 长春师范大学 生命科学学院， 长春 130032；
    3. 福建农林大学 生命科学学院， 福州 350002
  • 出版日期:2021-10-18 发布日期:2021-10-21
  • 通讯作者: 孙　刚，博士，教授，主要从事环境生态学研究，E-mail：sungang@nenu.edu.cn
  • 作者简介:房　岩，博士，教授，主要从事功能生物学研究，E-mail：fangyan124@aliyun.com
  • 基金资助:
    国家自然科学基金项目（31671010）； 吉林省自然科学基金项目（20180101280JC）； 吉林省教育厅“十三五”科学技术项目（JJKH20181167KJ）； 三明学院引进高层次人才科研启动项目（18YG02；18YG01）

Study on the blood compatibility of biomimetic polymer film mimicking the locust wing

  1. 1. School of Resources and Chemical Engineering，Fujian Provincial Medical Plant Exploitation and Utilization
    Engineering Research Center, Fujian Provincial Key Laboratory of Resources and Environment Monitoring &
    Sustainable Management and Utilization, Sanming University, Sanming 365004, China;
    2. School of Life Sciences, Changchun Normal University, Changchun 130032, China；
    3. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Online:2021-10-18 Published:2021-10-21

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摘要： 以蝗虫翅为模板、聚二甲基硅氧烷（PDMS）为基材，采用软刻蚀和二次转写法，制备高分子仿生膜，评价其血液相容性。结果表明，仿生膜成功复制了翅表面的形貌结构，表现出良好的疏水性（水滴接触角141.3°~147.6°）、疏血性（血液接触角119.8°~126.3°）和血液相容性。在以中华稻蝗、棉蝗和东亚飞蝗翅为模板的仿生膜表面，血小板黏附量分别为3.06×104、2.83×104和0.95×104 个/cm2，明显低于对照组（10.89×104 个/cm2）。其中，东亚飞蝗翅仿生膜表面的血小板数量最低，仅为对照组的8.7%，同时形态完好，未被激活，没有伪足、变形和聚集现象发生，具有较好的抗凝血性和更长的动态凝血时间。仿生膜表面溶血率均<5%，符合我国医用高分子材料的溶血率标准。多级层次结构促进血细胞与微单元之间的相互作用，微纳效应阻止血细胞的机械损伤，不利于血细胞的黏附和铺展，对凝血反应和微血栓形成具有显著的抑制作用。研究结果可为新型医用工程材料的制备提供新的思路和方法。

关键词: 蝗虫翅, 血液相容性, 微观结构, 高分子膜, 仿生

Abstract: Using locust wing as template and polydimethylsiloxane (PDMS) as substrate, biomimetic polymer films were prepared by means of soft etching and dual transcription, and the blood compatibility of the biomimetic film surface was evaluated. Based on the results, the micro-morphology of wing surface was replicated successfully on the biomimetic film, which exhibited high resistance against water (contact angle 141.3°-147.6°) and blood (contact angle 119.8°-126.3°) as well as fine blood compatibility. On the surface of biomimetic film mimicking the wing of Oxya chinensis, Chondracris rosea rosea and Locusta migratoria manilensis, the amount of platele adhesion were 3.06×104, 2.83×104 and 0.95×104 ind./cm2, respectively, remarkably lower than that (10.89×104 ind./cm2) on the CK film. On the surface of biomimetic film mimicking the wing of L. migratoria manilensis, the amount of platele adhesion was the lowest, just 8.7% of the CK; meanwhile, the platele kept intact form, was not activated, without pseudopodia, deformation, and aggregation, implying better anticoagulant function and longer dynamic coagulation time. On the surface of biomimetic film, the hemolysis rate was lower than 5%, complying with China’s standard for medical polymer materials. The multiple hierarchical structure improved the interaction of blood cells and micro unit. The micro-nano effect prevented blood cells from mechanical injuring, adhering, and spreading. The coagulation cascade and microthrombus formation were markedly inhibited. The results might provide novel idea and method for fabrication of innovative medical engineering materials.

Key words: locust wing, blood compatibility, microstructure, polymer film, biomimetic

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