生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (4): 114-.doi: 10. 3969 / j. issn. 2095-1736. 2021. 04. 114

• 技术方法 • 上一篇    下一篇

d(G3AG2T3G3AT)体外形成联锁型G-四链体结构和稳定性

  

  1. 1.安徽大学物质科学与信息技术研究院,合肥230601;2.中国科学院强磁场科学中心,合肥230031; 3.中国科学技术大学,合肥230026
  • 出版日期:2021-08-18 发布日期:2021-08-18
  • 通讯作者: 王　涛,副研究员,博士,研究方向为结构生物学和生物化学与分子生物学,E-mail:wangtaochmfl@hmfl.ac.cn
  • 作者简介:王　涛,副研究员,博士,研究方向为结构生物学和生物化学与分子生物学,E-mail:wangtaochmfl@ hmfl.ac.cn
  • 基金资助:
    国家自然科学基金资助项目(31700678);安徽省自然科学基金资助项目(1708085MC63)

Structure and stability of interlocked G-quadruplexes for medbyd(G3AG2T3G3AT)invitro

  1. 1. Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, China; 2. High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230031, China; 3. University of Science and Technology of China, Hefei 230026, China
  • Online:2021-08-18 Published:2021-08-18

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摘要： G-四链体是由富含连续鸟嘌呤碱基串联的DNA或RNA单链折叠而成,具有维持染色体稳定、调控转录和翻译及摘其他生物学进程的重要意义。能够形成G-四链体结构的潜在碱基序列广泛存在人类基因组和mRNA中,尤其是端粒及基因启动子处。主要研究序列d(G3AG2T3G3AT)分别在钠和钾离子条件下形成联锁型G-四链体结构的不同特性。结果表明:该序列在钾离子溶液中先形成了动力学有利结构,随后自发转换为具有超高热稳定性的热力学稳定结构;钠离子条件下预先形成的结构,经过后续钾离子滴定可以直接转换为更稳定的钾离子热力学稳定结构。由于联锁型G-四链体良好的水溶性、生物相容性以及超高的热稳定性,它在自组装纳米材料领域具有潜在的应用前景。

关键词: 联锁型G-四链体, 核磁共振, 圆二色谱, 稳定性, 纳米材料

Abstract: The single stranded DNA or RNA sequences having rich stretches of consecutive guanines can fold into four-stranded struc-tures known as G-quadruplexes, which play crucial roles in chromosomal maintenance, transcription and translation, as well as otherbiological processes. The putative G-quadruplex-forming sequences are wildly spread among the human genome and mRNA, especiallymore frequent in the telomere and promoters. Here, we focused on the different characteristics of the interlocked G-quadruplexes adopt-ed by sequence d(G3AG2T3G3AT) in the presence of sodium and potassium ions, respectively. The experimental results showed that d (G3AG2T3G3AT) at first folded into a kinetically favorable conformation in K+solution, and then spontaneously converted to a thermo-dynamically stable conformation with an extremely high thermal stability. Secondly, upon K+ion titration, the interlocked G-quadruplex structure even well pre-folded in an excessive amount of Na+still enabled a ready conversion to the thermodynamically stable conforma-tion ultimately stabilized by K+. This interlocked G-quadruplex will be expected applicable in the field of nanomaterial due to its con-siderable water-solubility, biocompatibility and extreme stability.

Key words: interlocked G-quadruplex, nuclear magnetic resonance (NMR), circular dichrosim(CD), stability, nanomaterial

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