生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (4): 118-.doi: 10.3969/j.issn.2095-1736.2024.04.118

• 教学研究 • 上一篇    下一篇

基于任务驱动的多糖制备工艺对其黏弹性影响综合实验教学设计

李　慧1， 王继乾1， 姬思雪1， 赵玉荣1， 马少华2， 陈翠霞1   

  1. 1. 中国石油大学（华东） 化学化工学院， 青岛 266580；
    2. 中国石油大学（华东） 石油工业训练中心， 青岛 266580
  • 出版日期:2024-08-18 发布日期:2024-08-14
  • 通讯作者: 陈翠霞，博士，教授，研究方向为细胞生物学，E-mail:chencx@upc.edu.cn
  • 作者简介:李慧，博士，讲师，研究方向为微生物代谢工程、基因工程、微生物多糖发酵工程，E-mail:lihui@upc.edu.cn
  • 基金资助:
    山东省教学改革项目重点项目（Z2022114）；中国石油大学（华东）校级教学改革重点项目（CZ2022007）；2021年中国石油大学（华东）校级探究性实验项目；国家自然科学基金项目（31800075）

Design of the comprehensive experiment “the effects of preparation methods on the properties of microbial polysaccharides”based on task-driving model

LI Hui1, WANG Jiqian1, JI Sixue1, ZHAO Yurong1, MA Shaohua2, CHEN Cuixia1   

  1. 1. College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao,
    266580, China; 2. China Petroleum Industry Training Center, China University of Petroleum (East China),
    Qingdao 266580, China
  • Online:2024-08-18 Published:2024-08-14

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摘要： 基于科教融合和成果导向（OBE）教育理念，将生化领域的前沿科技成果转化为教学实验，培养学生的科研创新素质。引入科研项目“多糖的制备工艺对其黏弹性能的影响”并贯穿教学全过程，引导学生查阅文献并讨论“耐温耐盐、环境友好新型驱油剂开发”的热点问题，拓宽学生的学术视野，培养学生文献查阅能力；鼓励学生根据自己的知识体系设计个性化实验方案，培养学生分析问题、解决问题的能力；指导学生探究利用盐醇析法和Sevage法制备多糖的工艺并计算其得率，采用红外和紫外光谱对样品进行表征，探究不同制备工艺对黏弹性及驱替效果的影响，帮助学生搭建“生物—化工—能源”多学科知识体系，提升实践创新能力；开展数据分析、方案评价和成果路演，锻炼学生的科研探究能力。

关键词: 任务驱动, 胞外多糖, 制备, 黏弹性, 综合性实验

Abstract: Based on the concept of “integration of science and education” and “outcome based education (OBE)”, the frontier scientific and technological achievements in the field of biochemistry were introduced into teaching experiments to cultivate students’ scientific research and innovation quality. The scientific research project “the effects of preparation methods on the properties of microbial polysaccharides” was introduced through the whole teaching process. Firstly, the students were guided to retrieve the literature and discuss the hot issues of “the development of a new type of environmentally friendly oil-displacing agent that was resistant to temperature and salt” to broaden their professional vision and cultivate their literature retrieving ability. Secondly, students were encouraged to design individualized experimental schemes according to their own knowledge system to cultivate their ability to analyze and solve problems. Thirdly, they prepared polysaccharide samples by salt-alcohol-precipitation method and Sevage method respectively, calculated the yield, characterized the samples by infrared spectra and ultraviolet spectra, and investigated the influence of different preparation methods on visco-elasticity and displacement effect of polysaccharides. This process helped students to establish their multidisciplinary knowledge system of “biology-chemical engineering-energy” and improved their experimental practice ability. Finally, the students carried out data analysis, program evaluation and achievement roadshow to train their scientific research ability.

Key words: task-driving model, exopolysaccharides, preparation methods, visco-elasticity, comprehensive experiment

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