生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (5): 82-.doi: 10.3969/j.issn.2095-1736.2022.05.082

• 研究报告 • 上一篇    下一篇

低氮胁迫下胡杨离子平衡和硝酸盐分配的调控响应

  

  1. 1.西南科技大学 生命科学与工程学院， 绵阳 621010；
    2. 新疆农业大学 林学与园艺学院， 乌鲁木齐 830052
  • 出版日期:2022-10-18 发布日期:2022-10-19
  • 通讯作者: 姚银安，博士，教授，研究方向为植物生理生态学，E-mail: yinanyao@swust.edu.cn
  • 作者简介:张林，硕士，研究方向为植物遗传与种质创新，E-mail: 2636461539@qq.com; 孙玉芳，博士，研究方向为树木生理，E-mail: 526947588@qq.com；张林和孙玉芳为并列第一作者
  • 基金资助:
    国家自然科学基金项目（31770644）；四川省科技计划项目（2021YJ0508）

Low nitrogen-induced ion balance and nitrate distributionin Populus euphratica Oliv.

  1. 1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010,
    China; 2. College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China
  • Online:2022-10-18 Published:2022-10-19

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摘要： 研究采用盆栽控制试验方法分析胡杨耐低氮策略。结果表明：胡杨植株碳氮比较高，维持较高的氮素利用率；低氮胁迫下，胡杨硝酸盐优先满足地上部；根系和地上部相反的硝酸盐同化限速酶（硝酸还原酶）活性变化促进了NO-3的地上部转运，承担木质部长距离硝酸盐运输NRT1.5C/NRT1.8B蛋白的转录水平改变进一步加快NO-3地上部运输；同时，承担老叶向幼叶转运硝酸盐蛋白PeNRT1.7转录水平提高，加速植株氮素营养周转。结果表明：低氮下根系一价阴离子NO-3和Cl-含量的大幅度降低，伴随着NO-3长距离运输的K+含量降低；导致二价离子SO2-4、Ca2+、Mg2+补偿性升高，从而保证植株离子平衡。因此，胡杨通过合理的离子分配和碳氮平衡策略有效地适应了中等氮素胁迫压力。

关键词: 低氮胁迫, 离子平衡, 硝酸盐长距离运输, 碳氮平衡

Abstract: The potted control experiment method was used to analyze Populus euphratica Oliv. strategies to tolerate low nitrogen. The results showed that: Populus euphratica Oliv. plants have high carbon-nitrogen ratio to maintain a high nitrogen utilization rate; under low nitrogen stress, Populus euphratica Oliv. nitrates preferentially meet the shoots; roots and shoots have opposite nitrate assimilation rate-limiting enzymes (nitrate reductase). The activity change promoted the NO-3 transportation of the shoots and the transcription level of the NRT1.5C/NRT1.8B protein, which was responsible for the long-distance nitrate transportation of the xylem, further accelerated the NO-3 transportation of the shoots. At the same time, it was responsible for the transportation of nitrate from old leaves to young leaves. The increasing transcription level of the protein PeNRT1.7 accelerated the plant nitrogen nutrient turnover. Result showed that the root monovalent anions NO-3 and Cl- content greatly decreased under low nitrogen, and the K+ content of NO-3 long-distance transportation was reduced, resulting in a compensatory increase in the divalent ions SO2-4, Ca2+, and Mg2+, thereby ensured the plant ion balance. Therefore, Populus euphratica Oliv. could effectively adapt to moderate nitrogen stress through reasonable ion allocation and carbon and nitrogen balance strategies.

Key words: low nitrogen stress, ionic homeostasis, long-distance transportation of nitrate, carbon and nitrogen balance

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