生物学杂志 ›› 2021, Vol. 38 ›› Issue (6): 54-.doi: 10.3969/j.issn.2095-1736.2021.06.054

• 研究报告 • 上一篇    下一篇

基于matK部分序列福建茶树遗传多样性分析

  

  1. 武夷学院 茶与食品学院,武夷山 354300
  • 出版日期:2021-12-18 发布日期:2021-12-15
  • 通讯作者: 李焰焰,教授,主要从事园艺学相关研究,E-mail: 253754917@qq.com
  • 作者简介:聂传朋,博士,主要从事生态学相关研究,E-mail: 361133372@qq.com
  • 基金资助:
    福建省自然科学基金项目(2019J01826);武夷学院高级引进人才科研启动项目(YJ201904、YJ202003)

Study on genetic diversity of tea tree from Fujian Province based on matK partial sequence

  1. College of Tea and Food Science,Wuyi University, Wuyishan 354300, China
  • Online:2021-12-18 Published:2021-12-15

摘要: 为研究福建茶树的遗传多样性水平,以福建省5个地区51个茶树品种为样本,分析其遗传结构和遗传分化。结果表明:福建茶树matK部分序列长度为833 bp,存在26个多态位点(S),7种单倍型(Hap1~Hap7),表现为高单倍型多样性(0.525)和低核苷酸多样性(0.001 89)。单倍体系统发育树形成4大分支,自展支持率均大于60%。不同茶树群体间的遗传距离为0.000 3~0.003 3,群体内遗传距离为0.000 0~0.003 7。福建茶树群体间分化系数Fst=-0.016 22~0.500 00和基因流Nm=-15.663 07~15.210 73。51个福建茶树的遗传多样性较低,不同群体之间存在一定程度的遗传分化。中性检验Tajima’s D、Fu and Li’D* and F*均为显著负值,错配分布曲线为单峰模式,表明福建茶树群体间发生了扩张事件。结果为进一步开发利用这一资源提供参考依据。

关键词: S571.1, Q941

Abstract: In order to study the genetic diversity of Fujian tea trees, the genetic structure and genetic differentiation of 51 cultivars of Fujian tea trees from 5 regions were analyzed. The results showed that the length of matK sequence of tea plants in Fujian was 833 bp, with 26 polymorphic sites (S) and 7 haplotypes (Hap1-Hap7), showing high haplotype diversity (0.525) and low nuclear glycoside diversity (0.001 89). The haploid phylogenetic tree formed 4 branches, and the self-expanding support rate was greater than 60%. The genetic distance between different tea populations was between 0.000 3 and 0.003 3, and the genetic distance within the population was between 0.000 0 and 0.003 7. The differentiation coefficient of Fujian tea tree population was Fst=-0.016 22-0.500 00 and gene flow was Nm=-15.663 07-15.210 73. The genetic diversity of 51 Fujian tea trees was low, and there was a certain degree of genetic differentiation between different groups. Neutral tests Tajima’s D, Fu and Li’D* and F* were significantly negative values, and the mismatch distribution curve was a unimodal pattern, indicating that expansion events occurred among the tea tree populations. The results of this study provided a reference for further development and utilization of this resource.

Key words: tea tree, matK partial sequence, genetic diversity

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