生物学杂志 ›› 2020, Vol. 37 ›› Issue (3): 121-.doi: 10.3969/j.issn.2095-1736.2020.03.121

• 教学研究 • 上一篇    下一篇

植物抗病基因的DNA改组在遗传学实验教学中的应用

  

  1. 南京大学 生命科学学院, 南京 210093
  • 出版日期:2020-06-18 发布日期:2020-06-10
  • 作者简介:张远莉,博士,讲师,研究方向为分子遗传学,E-mail:zhangyl@nju.edu.cn
  • 基金资助:
    南京大学生物学基地人才培养支撑条件建设(编号:J1210026)

Application of DNA shuffling of plant resistance genes in genetics experimental teaching

  1. School of Life Science, Nanjing University, Nanjing 210093, China
  • Online:2020-06-18 Published:2020-06-10

摘要: 遗传学实验是生命科学、医学、农学等相关领域的核心实验课程。在实验教学过程中,教师为激发学生学习兴趣,紧密结合当前科学研究,设计一些综合性、设计性实验,将科研成果引入课堂。综合性实验指导学生利用基因的DNA改组技术对植物抗病基因进行人工合成,从不同的水稻品种中克隆出基因片段,利用现存的自然变异,采用基因的DNA改组技术,得到新的基因片段,观察和了解基因的人工进化。可以让学生学习分子遗传学的基本操作,从分子水平揭示遗传学的基本现象与规律。与传统的遗传学实验相比,实验是综合性实验,具有一定生物学意义,通过综合性、设计性实验,培养学生初步独立进行科学研究的能力和创新能力,为创新型人才培养提供平台。

关键词: 遗传学实验, DNA改组, 人工进化;实验教学

Abstract: Genetics experiment is a core experiment course in life science, medicine, agriculture and other related fields. In order to stimulate students′ interest in learning genetics experiment, some genetics experiments about scientific research were designed in this class. In this comprehensive experiment, plant resistance genes were synthesized by DNA shuffling technology. Gene fragments were cloned from different rice varieties, and new genes were obtained by artificial evolution using the DNA shuffling technology. Through these comprehensive experiments, students can understand the basic operation of molecular genetics and the basic phenomena and laws of genetics at the molecular level. Compared with the traditional genetic experiment, students can also improve the ability to carry out scientific research. This will provide a platform for the cultivation of innovative talents.

Key words: genetics experiment, DNA shuffling, artificial evolution, experimental teaching

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