生物学杂志

生物学杂志 ›› 2020, Vol. 37 ›› Issue (2): 52-.doi: 10.3969/j.issn.2095-1736.2020.02.052

• 研究报告 • 上一篇    下一篇

大连典型浴场海水中粪源指示细菌和土著病原弧菌的流行分布

  

  1. 1. 国家海洋环境监测中心， 大连 116023； 2. 大连海洋大学 水产与生命学院， 大连 116023
  • 出版日期:2020-04-18 发布日期:2020-04-17
  • 通讯作者: 樊景凤，博士，研究员，主要研究方向为海洋生态学，E-mail: jffan@nmemc.org.cn
  • 作者简介:明红霞，博士，副研究员,主要从事海洋微生物学研究，E-mail: hxming@nmemc.org.cn
  • 基金资助:
    国家重点研发计划“全球变化及应对专项”(2016YFA0601401);国家海洋局近岸海域生态环境重点实验室基金(201813) 

Distribution of fecal indicator bacteria and indigenous pathogenic vibrio in the seawater of Dalian typical bathing beach

  1. 1. National Marine Environmental Monitoring Center, Dalian 116023; 2. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
  • Online:2020-04-18 Published:2020-04-17

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摘要： 粪便污染指示细菌可能不能反映海洋土著病原细菌的污染，因此，在游泳高峰期选取肠球菌、副溶血弧菌、创伤弧菌和霍乱弧菌为研究对象，分析了大连付家庄浴场和星海浴场细菌学指标的分布特征。结果表明，肠球菌的超标率为58.2%（n=134），副溶血弧菌、创伤弧菌和霍乱弧菌的毒力基因标志物检出率分别为24.6%、10.4%和4.5%（n=134），两个浴场不但受到了比较严重的粪便污染，而且均存在病原性副溶血弧菌和创伤弧菌，此外，付家庄浴场还存在病原性霍乱弧菌，对游客健康造成潜在威胁。风速、浪高、降雨对肠球菌影响较大，水温、气温、盐度、透明度是病原性创伤弧菌分布的主要影响因子，pH值、浪高对副溶血弧菌的分布影响较大。因肠球菌与弧菌的生态学特征差异较大，建议在现行浴场水质监测的基础上，增加常见病原弧菌的监测。

关键词: 肠球菌, 副溶血弧菌, 创伤弧菌, 霍乱弧菌, 浴场海水, 毒力基因标志物

Abstract: Fecal indicator bacteria may not reflect the pollution of marine indigenous pathogenic bacteria. Therefore, the distribution of bacteriological indicators, including enterococcus, Vibrio parahaemolyticus, V. vulnificus and V. cholerae was analyzed during the peak swimming season in Dalian Fujiazhuang and Xinghai bathing beachs. The results showed that the exceeding standard rate of enterococcus was 58.2% (n=134), and the detection rates of virulence gene markers of V. parahaemolyticus, V. vulnificus and V. cholerae were 24.6%, 10.4% and 4.5% (n=134), respectively. These two bathing beaches were not only polluted by fecal pathogens, but also existed the pathogenic V. parahaemolyticus and V. vulnificus, besides, the pathogenic V. cholerae was also detected in Fujiazhuang bathing beach, which all pose potential risks to human health. The speed of wind, wave height and rainfall had great impacts on the distribution of enterococcus. Water temperature, air temperature, salinity and transparency were the main factors affecting the distribution of pathogenic V. vulnificus. pH and wave height had great influences on the distribution of V. parahaemolyticus. Due to the different ecological characteristics of enterococcus and Vibrio spp., we suggested that important pathogenic vibrio should be considered into the current routine monitoring of bathing beach.

Key words: enterococci, V. parahaemolyticus, V. vulnificus, V. cholerae, beach seawater, virulence gene marker

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