关键词: 灰霉病, GC-MS, 番茄, 根系分泌物, 抗氧化酶活性

Abstract: Microbial control of soil-borne diseases is currently the most effective means of environmental protection. In this paper, GC-MS and bioassay were used to detect the growth rate, disease index, root exudates, and the effects of relative content and antioxidant enzyme activity of tomato plants induced by Pseudomonas choloeaphtis and Bacillus pumilus were studied. The results showed that two antagonists could directly inhibit the growth of Botrytis cinerea, and the induction of the two antagonists did not affect the growth rate of tomato plants, and its antioxidant enzyme activity was reduced. GC-MS detected nearly 30 root exudates. The contents of 2-keto-D-gluconic acid, 2-hydroxypropionic acid, malic acid, octadecanoic acid, linoleic acid, dodecanoic acid, terephthalic acid, methyl palmitate and ortho-benzene in the Pseudomonas choloeaphtis processing group and Bacillus pumilus processing group were significantly increased. In a comprehensive way, Pseudomonas choloeaphtis and Bacillus pumilus can not only directly inhibit the growth of limestone bacteria, when the role of tomato plants also have the role of inhibiting the pathogenesis of Botrytis cinerea, and can participate in the plant's own metabolism, and to protect the antioxidant enzyme activity of the plant itself and change the species and content of the secondary metabolites of plants to achieve the effect of disease resistance, effectively reduce oxidative damage in vivo.

Key words: Botrytis cinerea, GC-MS, tomato, root exudates, antioxidant enzyme activity