生物学杂志 ›› 2020, Vol. 37 ›› Issue (1): 50-.doi: 10.3969/j.issn.2095-1736.2020.01.050

• 研究报告 • 上一篇    下一篇

南沙海区深海沉积物中细菌多样性分析

  

  1. 1. 国家海洋环境监测中心, 大连 116023; 2. 国家海洋局近岸海域生态环境重点实验室,大连 116023; 3. 大连海洋大学 水产与生命学院, 大连 116023
  • 出版日期:2020-02-18 发布日期:2020-03-09
  • 通讯作者: 樊景凤,博士,研究员,主要研究方向为海洋生态学, E-mail: jffan@nmemc.org.cn
  • 作者简介:苏洁,博士,助理研究员,主要从事海洋微生物学研究, E-mail: jsu@nmemc.org.cn
  • 基金资助:
    国家重点研发计划“全球变化及应对专项”(2016YFA0601401);全球变化与海气相互作用专项(GASI-03-01-02-05);国家海洋局近岸海域生态环境重点实验室基金(201813)

Analysis on bacterial diversity in Nansha deep-sea sediments

  1. 1. National Marine Environmental Monitoring Center, Dalian 116023; 2. Key Laboratory for Ecological Environmental in Coastal Areas, State Oceanic Administration, Dalian 116023; 3. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
  • Online:2020-02-18 Published:2020-03-09

摘要: 为研究南沙海区深海沉积物中细菌多样性,采用16S rDNA克隆文库方法对南沙群岛3个深海站位沉积物中获得的565个阳性克隆子进行序列测定。发现3个站位所获的序列涵盖了变形菌门(Proteobacteria)、浮霉菌门(Planctomycetes)、放线菌门(Actinobacteria)、硝化螺旋菌门(Nitrospira)、绿屈挠杆菌门(Chloroflexi)、酸杆菌门(Acidobacteria)、螺旋体(Spirochaetes)、拟杆菌门(Bacteroidetes)、疣微菌门(Verrucomicrobia)和厚壁菌门(Firmicutes)共10个门。其中变形菌、浮霉菌和放线菌均为群落优势类群,这些优势类群组成与陆地以及海水中细菌优势类群存在显著差异,同时各站位细菌群落又表现出一定差异。上述结果表明南沙海区深海沉积物中具有丰富的细菌多样性,并且与其生存的环境密切相关。

关键词: 南沙, 深海沉积物, 细菌多样性

Abstract: To study bacterial diversity in Nansha deep-sea sediments, a total of 565 clones from three Nansha deep-sea sediments were sequenced using 16S ribosomal DNA (rDNA) clone library method. The results showed that the bacterial communities from three deep-sea sites covered 10 phyla: Proteobacteria, Planctomycetes, Actinobacteria, Nitrospira, Chloroflexi, Acidobacteria, Spirochaetes, Bacteroidetes, Verrucomicrobia and Firmicutes. The predominant group in these three deep-sea sediments included Proteobacteria, Planctomycetes and Actinobacteria, which was different from the dominant group in land and marine microbiology. The above results suggested that microbial diversity of Nansha marine sediments was rich.

Key words: Nansha, deep-sea sediments, bacterial diversity

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