生物学杂志

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铅胁迫对紫藤幼苗生长、生理指标及Pb2+富集的影响

  

  1. 江西财经大学 园林系, 南昌 330032
  • 出版日期:2019-02-18 发布日期:2019-02-18
  • 通讯作者: 江灶发,副教授,主要从事植物分类及其应用等方面研究,E-mail:1053324884@qq.com
  • 作者简介:周敏,硕士研究生,主要从事园林植物抗逆及应用研究,E-mail:422870517@qq.com
  • 基金资助:
    国家自然科学基金项目(21407069);江西省自然科学基金项目(2014BAB203024)

Effects of lead stress on growth, physiology, and lead ion accumulation and transportation in Wisteria seedling

  1. Department of Landscape Architecture, Jiangxi University of Finance and Economics, Nanchang 330032, China
  • Online:2019-02-18 Published:2019-02-18

摘要: 为探索紫藤对铅(Pb)胁迫的耐性生理响应,以紫藤(Wisteria sinensis)为材料,采用盆栽方式研究不同浓度(0、100、200、400、800和1000 mg/kg)Pb胁迫下,紫藤的生物量、生理指标变化和Pb的富集能力。试验结果表明,Pb<800 mg/kg对紫藤的生长及光合作用有一定的促进作用,但不显著(P>0.05),1000 mg/kg Pb处理时,仅地下部干、鲜重低于CK,Chlb含量显著低于CK(P<0.05);随Pb浓度升高,POD和CAT活性呈先增加后降低趋势,地上部SOD活性逐渐升高,除地下部CAT活性在1000 mg/kg Pb处理时低于CK,其他处理抗氧化酶活性均高于CK;AsA含量地上部呈上升趋势,地下部呈先升后降趋势,各处理地上部GSH含量均显著低于CK,地下部变化不明显;MDA含量仅1000 mg/kg Pb处理下,地下部显著增加36.06%。表明紫藤可通过体内抗氧化酶和相关物质的诱导及合成缓解Pb胁迫对其造成的毒害作用。此外,地上部Pb2+吸附量在800 mg/kg Pb处理下最高,达57.38 mg/kg,地下部在1000 mg/kg Pb处理时最高,达1131.42 mg/kg;运转系数随Pb浓度的升高逐渐下降。综合分析结果表明,紫藤具有一定的耐Pb特性、Pb富集能力和修复Pb污染土壤的潜能。

关键词: 紫藤, Pb胁迫, 生理抗性, 富集

Abstract: The effects of Pb treatments(0, 100, 200, 400, 800 and 1000 mg/kg)on the growth, physiological and biochemical response and Pb accumulation of Wisteria sinensis were studied by pot culture. The results showed that Pb <800 mg/kg had no significant effect on the growth and photosynthesis of W. sinensis (P>0.05), and only 1000 mg/kg Pb had lower dry weight than CK, and its Chlb content was significantly lower than CK(P<0.05); with the increase of Pb concentration, the activities of POD and CAT increased firstly and then decreased, and the activity of SOD in the shoots increased gradually. The activity of CAT in the underground was lower than that of CK in the treatment of 1000 mg/kg Pb, but the activities of antioxidant enzymes were higher than that of CK;AsA content in the aboveground part was of the upward trend. The underground part shoed the of trend the first rise and then down, and the contents of GSH in the aboveground treatments were significantly lower than that of CK, while the change of underground part was not obvious; MDA content in the underground part increased significantly by 36.06% only 1000 mg/kgPb treatment, meaning that W. sinensis can relieve the toxic action caused by Pb stress via induction and synthesis of antioxidant enzymes and related substances in vivo. The Pb content in the aboveground part under 800 mg/kg Pb was highest and reached to 57.38 mg/kg, and the highest Pb content in the underground part was obtained under 1000 mg/kg Pb treatment, of 1131.42 mg/kg. The values of translocation factor gradually decreased as the increasing of Pb concentration. The comprehensive analysis result indicated that W. sinensis possesses a certain resistance to Pb, Pb enrichment capability and the potential ability to repair contaminated soil by lead.

Key words: Wisteria sinensis, lead (Pb) stress, physiological resistance, accumulation

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