生物学杂志

生物学杂志

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基于线粒体COⅡ基因和D-loop区序列对江西安南光唇鱼（Acrossocheilus）物种鉴定及系统发育位置分析

  

  1. 宁波大学 应用海洋生物技术教育部重点实验室，宁波 315211
  • 出版日期:2019-02-18 发布日期:2019-02-18
  • 通讯作者: 李明云，教授，博士生导师，研究方向为鱼类遗传育种和种质资源保护，E-mail：limingyun@nbu.edu.cn
  • 作者简介:薛艳洁，硕士研究生，专业方向为水产养殖，E-mail：xueyanjie333@163.com
  • 基金资助:
    国家星火计划项目（2011AN701001）；浙江省科技厅重大科技专项遗传育种项目（2012C12907-8）；浙江省海洋与渔业项目（浙海渔计\[2012\]83号）

Species identification and phylogenetic position of Acrossocheilus fasciatus from Annan in Jiangxi Province based on mitochondrial COII and D-loop region sequences

  1. Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China
  • Online:2019-02-18 Published:2019-02-18

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摘要： 以采自江西赣州安南的野生鱼为实验材料，分别设计引物扩增其线粒体DNA COⅡ和D-loop序列并进行序列比对分析，经扩增得到的COⅡ序列全长为691 bp，D-loop序列的全长为937~944 bp，其中COⅡ序列中A、T、G、C的平均占比分别为31.00%、25.80%、15.30%和27.90%，而D-loop序列的占比则分别为35.60%、32.30%、12.00%和20.00%，两序列都是A+T含量高于G+C，G的含量在4种碱基中最低。COⅡ序列中仅存在6个碱基位点的转换，D-loop则有多个碱基位点存在转换、颠换、缺失或插入，群体内遗传距离分别为0.0075、0.0050。用最大似然法构建的COⅡ和D-loop系统发育树显示赣州安南光唇鱼与半刺光唇鱼（Acrossocheilus hemispinus）亲缘性最近，序列相似性分别为98.70%、98.69%，遗传距离最近分别为0.0130、0.0260，未达到种间分化的水平（0.05）。2个序列分析结果一致，结合外部形态特征观察，可以断定赣州安南光唇鱼与半刺光唇鱼为同一种，研究为江西赣州安南光唇鱼的资源利用提供了资料。

关键词: 光唇鱼, 形态特征, COⅡ, D-loop, 序列分析, 系统发育分析

Abstract: Using Acrossocheilus fasciatus from Annnan in Jiangxi province, China, as experimental materials, we analyzed the variability of mitochondrial cytochrome oxidase unit Ⅱ (COⅡ) gene and D-loop region, and discussed the phylogenetic positon and species identification of two kinds of A. fasciatus. The results showed that the COⅡ gene was 691 bp in length, and the D-loop gene was 931-944 bp in length. Regardless of COII, or D-loop, the content of bases A+T was higher than that of bases G+C. Only 6 base sites were transformed in COⅡ sequence, while several base sites in D-loop sequence were transformed, transversed, and insertions/deletions. The genetic distances within the population were 0.0075 and 0.0050, respectively. The COⅡ and D-loop phylogenetic tree constructed by the maximum likelihood method showed the closest relationship between JXAnnan and Arossocheilus hemispinus. The sequence similarities were 98.70% and 98.69%, respectively. The genetic distances were 0.0130 and 0.0260, respectively, indicating that the genetic distance did not reach the level of interspecific divergence (0.05). The results demonstrated that JXAnnan is a geographical group of A. hemispinus. The results of this study can provide information for taxonomic study of Acrossocheilus.

Key words: Acrossocheilus fasciatus, morphological character, COⅡ gene, D-loop region, sequence analysis, phylogenetic analysis

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