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细胞外液钙离子浓度的改变对大鼠比目鱼肌离体单一肌梭自发放电的影响

  

  1. 1. 西安医学院, 西安 710021; 2. 湖北文理学院 医学院机能学部, 襄阳 441053;3. 西安交通大学医学部, 西安 710016
  • 出版日期:2018-04-18 发布日期:2018-04-18
  • 通讯作者: 曹健,博士,副教授,研究方向为神经生理学,Email: littlegrass123@163.com
  • 作者简介:余蕾,讲师,硕士,研究方向为神经生理学,Email:yulei@xiyi.edu.cn
  • 基金资助:
    国家自然科学基金青年基金项目(31300981); 陕西省教育厅专项科研基金(2015JK1616)

Effects of extracellular calcium concentration on the spontaneous discharge of isolated rat muscle spindle

  1. 1. Xi′an Medical University, Xi′an 710021; 2. Hubei University of Arts and Science, Xiangyang 441053;3. Medical College, Xi′an Jiaotong University, Xi′an 710016, China
  • Online:2018-04-18 Published:2018-04-18

摘要: 观察了含有不同钙离子浓度的细胞外液对大鼠离体单一比目鱼肌肌梭自发放电的影响。实验分为低钙改良任氏液组和高钙改良任氏液组,首先采用空气隔绝法记录大鼠离体单一比目鱼肌肌梭自发放电情况,然后分别以相应改良任氏液灌流肌梭,并记录其自发放电和氯化琥珀胆碱所致的诱发放电的情况。给予低钙改良任氏液灌流肌梭后,其自发放电频率增加(P<0.01),自发放电的周期间隔缩短(P<0.01),给予氯化琥珀胆碱后诱发放电未见明显增加;给予高钙改良任氏液灌流肌梭后,其自发放电频率减少(P<0.01),自发放电的周期间隔延长(P<0.05),给予氯化琥珀胆碱后诱发放电未见明显增加。提示含有较低钙离子浓度的细胞外液可增加大鼠离体单一比目鱼肌肌梭自发放电,含有较高钙离子浓度的细胞外液可抑制该肌梭自发放电。为大鼠肌梭生理的研究及钙离子与失重性肌萎缩的实验研究提供了有意义的资料。

关键词: 钙离子, 单一肌梭, 自发放电, 大鼠

Abstract: The impulse activities of muscle spindles isolated from the rat soleus muscle were investigated under varying concentrations of external calcium. The experiments were divided into low calcium modified Ringer′s solution group and high calcium modified Ringer′s solution group, using air gap technique to record the spontaneous discharge activities of isolated muscle spindle. And then with administrations of the corresponding modified Ringer′s fluid, the changes of the spontaneous discharges and evoked discharges by Succinylcholine chloride were recorded. After administration of Modified Ringer′s solution with low calcium, the spontaneous discharges of single muscle spindle were significantly increased(P<0.01), the interval of spontaneous discharge was shortened(P<0.01). After administration of Succinylcholine chloride, evoked discharges were not significantly increased. After administration of Modified Ringer′s solution with high calcium, the spontaneous discharges of single muscle spindle were significantly decreased(P<0.01), the interval of spontaneous discharge was prolonged(P<0.05). After administration of Succinylcholine chloride, evoked discharges were not significantly increased. The extracellular fluid with a low concentration of calcium ions can increase the spontaneous discharges of single muscle spindle of rats′ soleus muscle, and the extracellular fluid containing high calcium concentration can inhibit the spontaneous discharges of single muscle spindle of rats′ soleus muscle.

Key words: Ca2+, single muscle spindle, spontaneous discharge, rats