生物学杂志

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四指马鲅胃肠道内分泌细胞免疫组织化学的定位#br#

  

  1. 1. 中国水产科学研究院 南海水产研究所 农业部南海渔业资源开发利用重点实验室, 广州 510300;2. 上海海洋大学 水产与生命学院, 上海 201306; 3. 茂名市金阳热带海珍养殖有限公司, 茂名 525444
  • 出版日期:2018-04-18 发布日期:2018-04-18
  • 通讯作者: 区又君, 研究员, 研究方向为鱼类生物学、发育生物学与水产养殖技术研究, E-mail: ouyoujun@126.com
  • 作者简介:谢木娇,硕士,研究方向为海水鱼类基础生物学与繁育,E-mail:zuolan521314@126.com
  • 基金资助:
    广东省“扬帆计划”引进创新创业团队项目(2016YT03H038);广东省省级科技计划项目(2017B020204002)

Immunohistochemical localization of endocrine cells in gastrointestinal tract of Eleutheronema tetradactylum

  1. 1. Key Laboratory for Exploitation & Utilization of Marine Fisheries Resource in South China Sea, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300;2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306;3. Maoming Jinyang Tropical Fish Breeding Co.Ltd., Maoming 525444, China
  • Online:2018-04-18 Published:2018-04-18

摘要: 利用SABC免疫组织化学技术,选用5-羟色胺(5-HT),生长抑素(SS),胃泌素(GAS),胰高血糖素(GLU),神经肽(NPY),胆囊收缩素(CCK-8)6种兔抗胃肠激素抗体对四指马鲅(Eleutheronema tetradactylum)消化道内分泌细胞进行鉴别和定位。结果显示,免疫阳性细胞按形态可分为开放型内分泌细胞和封闭型内分泌细胞。食道内未检测出任何一种阳性细胞。胃部只检测出GAS和SS阳性细胞,但GAS细胞仅限在幽门部,密度较少。胃部各区域分布极多的SS阳性细胞,幽门盲囊只检测出SS细胞,前肠道只检测到GAS细胞,中肠未检测出任何阳性细胞,后肠则有GAS和SS细胞的分布。整个消化道均未检测出5-HT、GLU、NPY和 CCK-8阳性反应的细胞。讨论了其可能的内分泌方式和生理功能。

关键词: 四指马鮁, 消化道, 胃肠激素, 内分泌细胞, 免疫组织化学

Abstract: Identification and localization of gastrointestinal hormone endocrine cells in the digestive tract of Eleutheronema tetradactylum were carried out by using immunohistochemistry method of Strept Avidin-Biotin-Complex, six types of antisera raised against rabbit hormones such as 5-hydroxytryptamine(5-HT), somatostatin(SS), gastrin(GAS), glucagon(GLU), neuropeptide Y(NPY) and cholecystokinin(CCK-8). The results showed that the immunoreactive cells could be divided into open type endocrine cells and enclosed type endocrine cells by morpha. No positive cells were detected in the esophagus. The gastrin(GAS) and somatostatin(SS) positive cells were detected in the stomach, but the GAS cells were confined to the pylorus with less density. Plenty of SS positive cells were detected in sections of the stomach, SS cells were detected only in pyloric caeca, GAS cells were found only in foregut, no any positive cells detected in midgut, and GAS and SS cells could be found in hindgut. No any of 5-HT, GLU, NPY and CCK-8 immunoreactive cells was detected throughout the digestive tract. The speculative endocrine pattern and physiological functions of this species were discussed.

Key words: Eleutheronema tetradactylum, digestive tract, gastrointestinal hormone, endocrine cells, immunohistochemistry