关键词: 猕猴桃, 种质资源, 扩增片段长度多态性, 遗传多样性

Abstract: The genetic diversities and genetic differences of 33 kiwifruit germplasm resources colleted from Hunan, Sichuan, Guizhou and Hainan province were analyzed using amplified fragment length polymorphism (AFLP) markers. The results showed that 683 electrophoretic bands were detected totally, of which 622 bands were polymorphic by using 23 pairs of primers, representing 91.07% of the total bands. The identification rates of 33 germplasms were up to 100%. Based on clustering analysis on the AFLP results by UPGMA, the dendrogram indicated that the similarity coefficients of 33 germplasms of kiwifruit ranged from 0.51-0.95, presented a higher level of diversity. Thirty-three germplasms could divide into 3 groups at the similarity coefficient of 0.70, with Actinidia chinensis and A. deliciosa clustered into the largest group, A. latifolia clustered into the second group and Actinidia valvata the third. Thirty-one germplasms in the first group were further divided into several subgroups according to the similarity coefficient of 0.82. Accessions within Actinidia chinensis and A. deliciosa had closely genetic relationship and would be clustered preferentially related to their geographical origin. Cross clustering relationship among intraspecific and interspecific were also observed in this group. The combination of AFLP and traditional classification could be used in germplasm evaluation and variety selection.

Key words: kiwifruit, germplasm, amplified fragment length polymorphism, genetic diversity