生物学杂志

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环境胁迫对西藏拟溞SOD、CAT活性和MDA含量的影响

  

  1. 1. 大连海洋大学 辽宁省水生生物学重点实验室, 大连 116023;2. 辽宁省海洋水产科学研究院, 大连 116023
  • 出版日期:2017-06-18 发布日期:2017-06-18
  • 通讯作者: 赵文,教授,博士生导师,主要从事水域生态学研究,E-mail:zhaowen@dlou.edu.cn
  • 作者简介:魏杰,副研究员,主要从事浮游生物实验生态学研究,E-mail:weijie@dlou.edu.cn
  • 基金资助:
    国家自然科学基金项目 (41501535);国家科技基础性工作专项(2014FY210700)

Effects of environmental stress on activity of SOD, CAT and MDA content in Daphniopsis Tibetana Sars

  1. 1. Key Laboratory of Hydrobiology in Liaoning Province, Dalian Ocean University, Dalian 116023;
     2. Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China
  • Online:2017-06-18 Published:2017-06-18

摘要: 采用实验生态学方法,研究了高盐(20和25)和高温(20℃和25℃)胁迫对西藏拟溞幼溞和成溞的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量的变化。结果发现:1)在高盐胁迫下,幼溞的SOD、CAT活性增强,MDA含量降低;成溞的SOD活性变化不明显,CAT活性和MDA含量均降低。2)在高温胁迫下,幼溞的SOD、CAT活性显著增强,MDA含量也显著增加;成溞的SOD、CAT活性增强,MDA含量则降低。研究表明幼溞对高盐胁迫的应激能力较强,而对高温胁迫的应激能力较弱,成溞则相反。

关键词: 环境胁迫, 西藏拟溞, 超氧化物歧化酶, 过氧化氢酶, 丙二醛

Abstract: The present study investigated the effects of salinity (20 and 25) and temperature (20℃ and 25℃) stress on activities of SOD(Superoxide Dlismutase), CAT(Catalase) and MDA(Maldondialdehyde) content in juvenile and adult of Daphniopsis tibetana Sars, by method for experimental ecology, respectively. The result showed that high concentration of salt stress treatment increased the activities of SOD and CAT and reduced MDA content in juvenile. There were no significant differences in adult SOD activity induced by high salinity stress. And the activity of CAT and MDA content were higher in experimental group (salinity 20 and 25) compared with the control group (salinity 16). High temperature stress (20℃ and 25℃) obviously increased the activities of SOD and CAT, MDA content in juvenile. While the adult under high temperature treatment showed the increase SOD and CAT activities, yet the decrease of MDA content. The results indicated that the juveniles were more tolerant to high salinity stress, less tolerant to high temperature stress than the adults. The results were opposite in adults compared with juveniles under high salinity or temperature stress. These results provide some basic data for the research on antioxidative enzyme system in other cladocerans species under environmental stress, and scientific base for the domestication and large-scale culture of Daphniopsis tibetana Sars.

Key words: environmental stress, Daphniopsis tibetana Sars, SOD, CAT, MDA