生物学杂志

生物学杂志

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热休克蛋白70-甲胎蛋白抗原表位肽疫苗抗小鼠H22肝癌细胞免疫机制的研究

  

  1. 1. 陕西中医药大学 免疫及检验学教研室， 咸阳 712046； 2. 西安市中心医院 感染科， 西安 710003
  • 出版日期:2017-04-18 发布日期:2017-04-18
  • 通讯作者: 王巧侠，硕士，副主任医师，主要从事消化系统疾病临床与基础研究，E-mail: 894449620@qq.com
  • 作者简介:胥冰，硕士，副教授，主要从事分子免疫学研究工作,E-mail：xubing-95@163.com
  • 基金资助:
    国家自然科学基金（No.81172135）；陕西省自然科学基础研究项目(No.2016JM8023，2016JM8150)；陕西省教育厅自然科学基础研究项目( No.07JK233，14JS025)

Study of anti-tumor immunity elicited by peptides complex- heat shock protein 70 and alpha-fetoprotein epitope peptide

  1. 1. Immunology and the Science of Inspection Teaching and Research Section, Shaanxi Universit甲胎蛋白 (AFP)；热休克蛋白70( HSP70)；多肽疫苗；抗瘤免疫y of Chinese Medicine,Xianyang 712046； 2. Department of Infectious Disease, Xi′an Central Hospital, Xi′an 710003, China
  • Online:2017-04-18 Published:2017-04-18

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摘要： 分别以AFP多肽、HSP70 和HSP70-AFP多肽复合物免疫小鼠，检测各组CD8+T细胞培养上清IFN-γ、TNF-α、穿孔素、颗粒酶B水平及CD8+T细胞对H22细胞的杀伤作用，探讨热休克蛋白70(HSP70)-甲胎蛋白(AFP)抗原表位肽复合物对小鼠H22肝癌细胞的免疫效应。

关键词: 甲胎蛋白 (AFP), 热休克蛋白70( HSP70), 多肽疫苗, 抗瘤免疫

Abstract: To study anti-tumor immunity mechanism induced by a reconstructed peptide complex-heat shock protein 70 (HSP70) and alpha-fetoprotein (AFP), mice were immunized with recombinant peptide vaccine HSP70-AFP-P, whereas AFP peptide or HSP70 vaccination as controls. IFN-γ, TNF-α and granzyme-B, perforin-produced by separated CD8+ T cells from vaccinated mice were detected with ELISA, respectively. In vivo tumor loaded test was exerted to evaluate the immune effect of the peptide vaccine. The study suggested that sequential immunization with HSP70-AFP peptide vaccine could bring about effective AFP-specific CD8+ T cell responses and elicit specific antitumor immunity on AFP-producing tumors.

Key words: alpha-fetoprotein(AFP), heat shock protein 70 (HSP70), peptide vaccine, tumor immunity