生物学杂志

生物学杂志

• 技术方法 • 上一篇    下一篇

杜仲内生细菌DZBG07对小麦全蚀病的生防作用

  

  1. 1.安徽农业大学植物保护学院 合肥230036; 2. 安徽农业大学农业园管理中心 合肥230036
  • 出版日期:2015-12-18 发布日期:2015-12-18
  • 通讯作者: 马元山(1968-)，男，安徽合肥人，高级农艺师，硕士。研究方向：植物生理学。(电话)0551-65786312；(电子邮箱)dingting98@126.com
  • 作者简介: 成静(1994-)，女，山西榆次人，。研究方向：植物病理学。(电话)0551-65786312；(电子邮箱)18355433@qq.com
  • 基金资助:
    安徽农业大学稳定和引进人才项目；安徽农业大学大学生创新创新基金项目

Biocontrol Effect of Endophytic Bacteria DZBG07 from Eucommia ulmoides Oliver on Wheat Take-all

  1. 1.School of Plant Protection, Anhui Agricultural University, Hefei 230036，China
  • Online:2015-12-18 Published:2015-12-18

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摘要： 本实验以获得的一株对小麦全蚀病菌有较好抑菌活性的杜仲内生细菌DZBG07为试验菌株，研究其对小麦全蚀病的防治效果，分析拮抗细菌DZBG07对不同生育期的小麦植株根部中多酚氧化酶 ( PPO )、过氧化物酶（POD )和苯丙氨酸解氨酶 ( PAL )活性的影响。结果表明，杜仲内生细菌DZBG07能增强了小麦对全蚀病的抗性, 其苗期小麦全蚀病的发病率仅为26.57 %，明显低于未经DZBG07处理的小麦全蚀病的发病率(（86.35 %)；且经杜仲内生细菌DZBG07处理的小麦植株，在不同生育期其根部的多酚氧化酶 ( PPO )、过氧化物酶（POD )和苯丙氨酸解氨酶 ( PAL )三种防御酶活性均有所提高。杜仲内生细菌DZBG07对小麦全蚀病的抗性研究为揭示其生防机理与应用该菌提供了科学依据。

关键词: 内生细菌, 小麦全蚀病, 多酚氧化酶, 过氧化物酶, 苯丙氨酸解氨酶

Abstract: In the study, the endophytic bacteria DZBG07 showing strong activity against wheat Gaeumannomyces graminis was researched for its the control effect to wheat take-all, and the effect of inoculation of DZBG07 in the wheat rhizosphere on related enzyme activity of wheat roots in different growth stages was analysed through pot experiments. The results indicated inoculation of DZBG07 in the wheat rhizosphere reduced significantly the incidence of wheat take-all, the incidence of wheat take-all by DZBG07 was 26.57%, which was lower than that of the normal control groups(86.35%), and the activities of Polyphenol oxidase, Peroxidase and L-phenylalanin ammo-lyase of wheat roots treated with of DZBG07 were significantly higher than that of normal control groups. The resistance of DZBG07 to wheat take-all afforded scientific basis for its application and the further research on bio-controlling mechanism.

Key words: Endophytic bacteria, Wheat take-all, Polyphenol oxidase: Peroxidase: L-phenylalanin ammo-lyase