生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (2): 33-.doi: 10.3969/j.issn.2095-1736.2025.02.033

• 研究报告 • 上一篇    下一篇

产细菌素乳酸菌Lpe59的选育及其细菌素基因簇的挖掘

张宗源1， 岳丹丹1， 周留柱1， 王雪妍1， 陈登辉1， 潘梦诗1， 徐宏光1， 李冠杰1， 尹文兵2
  

  1. 1. 河南省科学院生物研究所有限责任公司， 郑州 450008；
    2. 中国科学院微生物研究所真菌学国家重点实验室， 北京 100101
  • 出版日期:2025-04-18 发布日期:2025-04-14
  • 通讯作者: 尹文兵，博士，研究员，研究方向为微生物次级代谢，E-mail:yinwb@im.ac.cn
  • 作者简介:张宗源，硕士，助理研究员，研究方向为食品微生物，E-mail:xuanyizzy@163.com
  • 基金资助:
    河南省科技攻关项目(232102110100)； 河南省科学院杰出青年人才培养项目(210405010)； 河南省科学院基本科研业务费项目（240605014）； 河南省科学院省级特聘研究员项目（230505002）

Screening of bacteriocin-producing lactic acid bacteria Lpe59 and mining of bacteriocin gene cluster

ZHANG Zongyuan1, YUE Dandan1, ZHOU Liuzhu1, WANG Xueyan1, CHEN Denghui1,PAN Mengshi1, XU Hongguang1, LI Guanjie1, YIN Wenbing2   

  1. 1. Henan Academy of Science Institute of Biology Co., Ltd., Zhengzhou 450008, China;
    2. State Key Laboratory of Myology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
  • Online:2025-04-18 Published:2025-04-14

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摘要： 为了筛选一株产细菌素乳酸菌，并对其进行细菌素基因簇的挖掘，以大肠杆菌、金黄色葡萄球菌为指示菌，通过常规方法初筛和复筛后筛选出1株抑菌能力较强的产细菌素的戊糖乳杆菌Lpe59。全基因组测序结果显示，菌株Lpe59基因组大小为3274558 bp，编码3047个基因。在COG、GO、KEGG、CAZy和VFDB数据库中分别注释到2434、2203、1614、107和4个功能基因。应用在线软件AntiSMASH 6.11预测到该菌具有核糖体合成和翻译后修饰肽类（RiPP-like）、Ⅲ型聚酮（T3PKS）化合物、萜烯（terpene）和cyclic-lactone-autoinducer等4种次级代谢物基因，通过Bagel4预测到一条合成Ⅱ类细菌素Plantaricin K和细菌素Plantaricin EF基因簇。通过抑菌试验和基因组预测表明戊糖乳杆菌Lpe59是一株极具生防潜力的产细菌素乳酸菌，研究结果为其细菌素Plantaricin K和Plantaricin EF进一步开发应用提供理论基础。

关键词: 戊糖乳杆菌, 细菌素, 抑菌, 全基因组, 合成基因簇

Abstract: In order to screen a bacteriocin-producing lactic acid bacteria and explore its bacteriocin gene cluster, the pathogensEscherichia coliandStaphylococcus aureuswere used as indicator, through initial and repeated screenings using conventional methods,Lactobacillus pentosusLpe59, a strain with strong antibacterial activity and bacteriocin production was isolated. The whole-genome information of strain Lpe59 was obtainde through high-throughput sequencing, the results showed the genome size of strain Lpe59 was 3274558 bp, encoding 3047 genes, with 2434, 2203, 1614, 107 and 4 functional genes annotated in COG, GO, KEGG, CAZY and VFDB databases, respectively. Using the online software AntiSMASH 6.11, four types of secondary metabolite gene clusters were predicted in strain Lpe59, including ribosomally synthesized and post-translationally modified peptide-like (RiPP-like) compounds, type Ⅲ polyketide synthase (T3PKS) metabolites, terpene, and cyclic-lactone-autoinducer. Additionally, a gene cluster for the synthesis of class Ⅱ bacteriocins Plantaricin K and Plantaricin EF was predicted through Bagel4. In conclusion, antibacterial tests and genomic predictions suggested thatL.pentosusLpe59 was a promising lactic acid bacterium with biocontrol potential and the ability to produce bacteriocins. The research findings provided a theoretical basis for the further development and application of its bacteriocins, Plantaricin K and Plantaricin EF.

Key words: Lactiplantibacillus pentosus, bacteriocin, bacteriostasis, whole genome, synthetic gene cluster

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