生物学杂志 ›› 2021, Vol. 38 ›› Issue (6): 45-.doi: 10.3969/j.issn.2095-1736.2021.06.045

• 研究报告 • 上一篇    下一篇

果桑菌核病防治用药对家蚕生长发育的影响

  

  1. 1. 湖北省农业科学院 经济作物研究所,武汉 430070;
    2. 湖北省农业科学院 果树茶叶研究所,武汉 430070
  • 出版日期:2021-12-18 发布日期:2021-12-15
  • 通讯作者: 陈登松,研究员,主要从事家蚕品种选育,E-mail: hbcds816@126.com
  • 作者简介:吴凡,博士研究生,副研究员,主要从事家蚕资源的创新利用研究,E-mail: wufan996@163.com
  • 基金资助:
    湖北省农业科技创新项目蚕桑资源产业化利用关键技术研究(No.2019-620-000-001-09)

Effects of the medicines for preventing and controlling Sclerotinia sclerotiorum on growth and development of silkworm

  1. 1. Industrial Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430070, China;
    2. Fruit Tree and Tea Institute, Hubei Academy of Agricultural Sciences, Wuhan 430070, China
  • Online:2021-12-18 Published:2021-12-15

摘要: 为探讨果桑菌核病防治用药对家蚕生长发育的影响,对二龄起蚕分别添食多菌灵可湿性粉剂、腐霉利可湿性粉剂和甲基硫菌灵可湿性粉剂,持续添食至上蔟结茧,添食清水作为对照,分析添食对家蚕生长发育、龄期经过、五龄盛食期体重、茧质成绩的影响,并测定了五龄第4天幼虫中肠CAT和POD酶的活性。结果发现:添食甲基硫菌灵可湿性粉剂的家蚕幼虫无法完成世代发育,在二龄期死亡;添食多菌灵可湿性粉剂和腐霉利可湿性粉剂的家蚕可正常发育至上蔟结茧;添食多菌灵可湿性粉剂的家蚕龄期经过缩短,而腐霉利可湿性粉剂的家蚕龄期经过增加;添食多菌灵和腐霉利可湿性粉剂的家蚕体重均变小,对家蚕的茧质成绩造成一定的影响,五龄第4天家蚕中肠内CAT和PAD酶活力均升高。因此,在果叶兼用桑园菌核病的防治用药中,应禁止使用甲基硫菌灵可湿性粉剂,可用多菌灵可湿性粉剂和腐霉利可湿性粉剂进行防治。

关键词: 果桑菌核病, 家蚕, 多菌灵, 甲基硫菌灵, 腐霉利

Abstract: This study explored the effects of three mulberry sclerotium control drugs on silkworm growth and development. The second-instar (2nd) silkworms were fed with carbendazim, procymidone and thiophanate-methyl until the cocoon stage, respectively, taken fresh water as a control. The effects on the silkworm growth and development were measured by the length of larval stage, the body weight of the fifth instar, and the cocoon quality. Also the activity of antioxidant enzymes in the midgut of the fifth instar larvae on the fourth day was measured by CAT and POD activity kits. The larvae fed with thiophanate-methyl could not complete the generational development, which died at the 2nd instar. The silkworms fed with carbendazim and procymidone could normally develop into cocoon stage. However, compared that with the control group, the larval age of silkworms fed with carbendazim had shortened, while the age of silkworms with procymidone had increased. The weight of silkworms fed with carbendazim and procymidone became lighter, which suggested that these two powders had a negative effect on the cocoon performance of silkworms. The activities of CAT and POD enzymes in the midgut of silkworms had increased. Therefore, for the control of mulberry sclerotium, the usage of thiophanate-methyl should be prohibited, while carbendazim and procymidone might be used.

Key words: Sclerotinia sclerotiorum, Bombyx mori, carbendazim, thiophanate-methyl, procymidone

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