生物学杂志

生物学杂志 ›› 2020, Vol. 37 ›› Issue (2): 33-.doi: 10.3969/j.issn.2095-1736.2020.02.033

• 研究报告 • 上一篇    下一篇

内源性MHCⅠ类分子对海马神经元突起生长的影响

  

  1. 安庆师范大学 生命科学学院 皖西南生物多样性研究与生态保护安徽省重点实验室, 安庆 246133
  • 出版日期:2020-04-18 发布日期:2020-04-17
  • 作者简介:吕丹，博士，讲师，研究方向为神经-免疫学，E-mail: lvdan212@163.com
  • 基金资助:
    安徽省高校自然科学研究重点项目(KJ2016A433) 

The role of endogenous MHCⅠon the outgrowth of hippocampal neurons

  1. The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui,College of Life Sciences, Anqing Normal University, Anqing 246133, China
  • Online:2020-04-18 Published:2020-04-17

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摘要： 大量研究证实，在免疫细胞发育及免疫应答等过程中行使重要功能的免疫分子主要组织相容性复合体Ⅰ（MHCⅠ）类蛋白，在中枢神经系统神经元上有广泛表达，并与突触形成、精确突触连接的建立密切相关。为进一步探究MHCⅠ类分子在中枢神经系统中的功能，以体外培养小鼠海马神经元为对象，检测发现在海马神经元突起生长、突触形成前已有MHCⅠ类分子表达，推测其表达可能与神经元突起生长相关；通过特异性抗体阻断MHCⅠ类分子信号通路或敲除编码MHCⅠ类分子重链的基因，神经元突起数目显著增多；利用特异性抗体阻断在免疫系统中作为MHCⅠ类分子配体发挥作用的PirB分子信号通路，同样发现神经元突起数目显著增加。实验结果初步证实内源性表达MHCⅠ类分子具有限制海马神经元突起生长的功能，为深入研究MHCⅠ类分子在中枢神经系统的新功能及其作用机制奠定基础。

关键词: MHCⅠ, 海马神经元, 突起生长

Abstract: Accumulating evidence indicates that immune proteins major histocompatibility complex I (MHCⅠ) molecules are expressed by healthy neurons. Based on the study of related knockout animals, the neuronal MHCⅠ molecules have been proved to play important roles in synaptic formation, remodeling and plasticity, which are significantly different from their well-acknowledged immune functions. Using cultured hippocampal neurons, we show MHCⅠ proteins are expressed before neurite outgrowth. Knockout of MHC class I in cultured neurons or blocking of MHCⅠmolecules by MHCⅠ antibody increases the branch number of neurons. Blocking of pirB, which works with MHCⅠin immune system, mimics the phenotype of increased dendritic branching. Our results imply that endogenous MHCⅠplays a role in limiting the neurite outgrowth during the development of hippocampal neurons.

Key words: MHCⅠ, hippocampal neurons, neurite outgrowth

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