生物学杂志

生物学杂志

• 研究报告 • 上一篇    下一篇

掌叶覆盆子套种对板栗林土壤细菌群落结构演变及多样性的影响

  

  1. 1. 中国林科院亚热带林业研究所, 杭州 311400； 2．浙江省林木育种技术研究重点实验室,杭州 311400； 3. 浙江省金华武义县林业科技推广站, 金华 321200；4. 浙江万寿康生物科技有限公司, 金华 321200
  • 出版日期:2019-06-18 发布日期:2019-06-18
  • 通讯作者: 龚榜初，研究员，研究方向为经济林良种选育与高效栽培，E-mail：gongbc@126.com
  • 作者简介:徐阳，博士，助理研究员，研究方向为经济林良种选育与高效栽培，E-mail：xuyang198610@163.com
  • 基金资助:
    浙江省公益技术研究农业项目“板栗药材高效复合经营技术研究示范（2015C32072）”

Influence of inter-planting with Rubus chingii on Castanea mollissima soil bacterial structure and diversity

  1. 1. Research lnstitute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400; 2. Key Laboratory of Tree Breeding of Zhejiang Province, Hangzhou 311400;3. Forestry Science and Technology Promotion Station of Wuyi County, Jinhua 321200; 4. Wanshoukang Biological Technology Co., Ltd, Jinhua 321200, China
  • Online:2019-06-18 Published:2019-06-18

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摘要： 通过研究掌叶覆盆子套种对栗林土壤细菌群落结构及多样性的影响，为科学评价该种植模式的生态风险提供科学依据。采集不同掌叶覆盆子套种密度（0、100和400株/亩）栗林土样，进行土壤主要化学指标及土壤细菌类群测定与分析。结果表明：不同模式间板栗林土壤细菌组成较一致，变形菌门（31.20%）与酸杆菌门（29.70%）为最多门类细菌。套种掌叶覆盆子后，土壤细菌Chao1指数显著提高，而Simpson指数显著提高，土壤细菌优势度增加，但多样性下降。套种和套种密度对土壤细菌群落的影响分别主要表现在门水平与目水平。板栗林套种掌叶覆盆子后，土壤有机质含量、全氮含量、水解性氮含量、有效磷含量、全磷含量显著增加。而土壤钾元素随套种密度提升呈现显著下降趋势。土壤理化性质的改变在一定程度上解释板栗-掌叶覆盆子模式中细菌群落结构变化，其中酸杆菌门、酸杆菌目与土壤氮含量变化相关性最大，绿弯菌门的大幅度减少可能同土壤中有机质增加有关。

关键词: 板栗, 掌叶覆盆子, 套种, 土壤细菌

Abstract: In order to provide a scientific basis for evaluating the management measure of Castanea mollissima inter-planting with Rubus chingii, the impact of soil bacterial structure and biodiversity was studied. Soil samples from Castanea mollissima inter-planting with different density of Rubus chingii (0, 100 and 400 plants/Mu）were collected. Soil bacteria were sequenced by Illumina High Throughput Sequencing technique. And, the soil physical and chemical indexes were also analyzed. The results showed that soil bacterial structure of Castanea mollissima forest was similar among different modes, Proteobacteria (31.20%) and Acidobacteria (29.70%) were the most common bacteria. After inter-planting with Rubus chingii, Chao1 and Simpson index indicated dominance of soil bacteria were increased significantly, but diversity was decreased. The influences of inter-planting and density were mainly affected by soil bacterial community on the Phylum level and Order level, respectively. Castanea mollissima forest soil organic matter, total nitrogen, hydrolyzable nitrogen, available phosphorus and total phosphorus content were increased significantly by inter-planting with Rubus chingii. The soil potassium content was decreased with the increase of inter-planting density. The changes of soil physical and chemical property caused by inter-planting may explain the influence of bacterial community structure. The changes of Acidobacteria and Acidobacteriales were correlated with nitrogen content. The change of Chloroflexi was correlated with organic matter. 

Key words: Castanea mollissima, Rubus chingii, inter-plant, soil bacteria

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