生物学杂志

生物学杂志

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蛹虫草高产虫草素复合诱变育种及固态发酵优化

  

  1. 安徽大学 资源与环境工程学院， 合肥 230601
  • 出版日期:2019-04-18 发布日期:2019-04-18
  • 通讯作者: 王钰，教授，主要从事植物及微生物方面研究，E-mail: 03136@ahu.edu.cn
  • 作者简介:孙翠，硕士，研究方向为农业生态系统与种质资源利用，E-mail: 18856961719@163.com
  • 基金资助:
    安徽省科技攻关项目(11010302109)

Screen high-producing cordycepin by Cordyceps militaris through composite mutagenesis and optimizaation of solid state fermentation

  1. School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China
  • Online:2019-04-18 Published:2019-04-18

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摘要： 已成功选育出1 株高产虫草素诱变菌株C1-7，以蛹虫草C-8为出发菌株，采用60Coγ-UV复合诱变，并通过Design-expert法对其固态发酵条件进行优化分析。结果表明，前期温度为24℃、后期温度为21℃、初始含水量为64%的条件下，虫草素的产量最高可达9.595 mg/g，相对于出发菌株提高132.9%。通过12代传代培养后，遗传稳定性良好，并且通过栽培得到诱变菌株子实体虫草素产量比出发菌株提高了100.2%，该菌株具有潜在的应用价值。

关键词: 虫草素, 复合诱变, 蛹虫草, 响应面

Abstract: A cordycepin high-yielding mutants had been screened. The original strain Cordyceps militaris producing cordycepin was treated with 60Co-γ- UV irradiation and its fermentation conditions were optimized for cordycepin production.The results showed that solid state fermentation conditions were initial temperature 24℃, late temperature 21℃. Under these conditions, the yield of cordycepin reached 9.595 mg/g, which was 132.9% higher than that of original strains. The strain was continuously sub-cultured to the 12 generation and remained steady after subculture, in addition, 100.2% was improved by cultivation of the mutagenesis strain. In conclusion, a high cordycepin-producing strain C1-7 was successfully screened, which has potential application value in practice.

Key words: cordycepin, combined mutation, Cordyceps militaris, response surface methodology

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