生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (1): 83-.doi: 10.3969/j.issn.2095-1736.2021.01.083

• 研究报告 • 上一篇    下一篇

盐度胁迫对缢蛏血液离子浓度及碳酸酐酶基因表达的影响

  

  1. 1. 浙江万里学院 生物与环境学院 浙江省水产种质资源高效利用技术研究重点实验室， 宁波 315100；  2. 浙江万里学院 宁海海洋生物种业研究院，宁波 315100； 3. 宁波大学 海洋学院， 宁波 315100
  • 出版日期:2021-02-18 发布日期:2021-02-22
  • 通讯作者: 何琳，副教授，博士，研究方向为贝类养殖，Email：hlwithyou@qq.com
  • 作者简介:陈铭，硕士研究生，研究方向为海洋生物种质工程，E-mail:1257620359@qq.com
  • 基金资助:
    国家自然科学基金项目（31802322）；宁波市2015年度科技富民项目（2015C10008）；浙江省农业新品种选育重大科技专项（2016C02055-9）；浙江省农业新品种选育重大科技专项（2016C02055-9）；宁波市一流学科“环境科学与工程”

Effects of salinity stress on blood ion concentration and carbonic anhydrase gene expression in Sinonovaulaconstricta#br#

  1. 1. Key Laboratory of Aquatic Germplasm Resource of Zhejiang Province, School of Biology and Environment,Zhejiang Wanli University, Ningbo 315100, China; 2. Ninghai Institute of Marine Biology Seed Industry,Zhejiang Wanli University, Ningbo 315100, China; 3. School of Marine Sciences,Ningbo University, Ningbo 315100, China
  • Online:2021-02-18 Published:2021-02-22

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摘要： 比较缢蛏在5种盐度（盐度5、15、20、25和35）的水体中胁迫不同时间（4、8、12、24、48、72和96 h）后，血液中Na+、K+和Cl－浓度变化以及碳酸酐酶 (carbonic anhydrase，CA) 基因mRNA表达变化。结果显示：随盐度升高，Na+、K+和Cl－浓度呈上升趋势。随时间推移，低盐组Na+、K+和Cl－浓度呈先下降、后稳定的变化趋势；高盐组Na+、K+和Cl－浓度呈先上升、后稳定的变化趋势。qPCR结果显示，CA基因在各个组织中均有表达，鳃中表达量最高。盐度胁迫后各个实验组的CA基因mRNA表达水平随着时间的推移均呈先上升后下降，最后趋于平稳的状态，并在24 h达到峰值。盐度5与盐度35实验组的CA mRNA表达水平比盐度15与盐度25实验组的变化趋势更为明显。实验结果表明缢蛏在盐度胁迫下血液Na+、K+和Cl－浓度及CA基因mRNA表达量均发生变化，迅速调节体内外离子浓度来维持渗透压的平衡。实验研究了离子浓度以及CA基因在缢蛏急性盐度胁迫过程中的作用，为缢蛏在适应盐度环境变化的分子生理机制提供基础资料。

关键词: 盐度, 离子浓度, 碳酸酐酶, 缢蛏

Abstract: tNa+, K+ and Cl－ concentrations in blood and mRNA expression of carbonic anhydrase (CA) gene in Sinonovaulaconstricta exposed to five salinity (salinity 5, 15, 20, 25 and 35) at different time (4, 8, 12, 24, 48, 72 and 96 h) were measured.The results showed that: the concentrations of Na+, K+ and Cl－ in the blood of sinonovaulaconstricta increased with the rise of salinity. The concentrations of Na+, K+ and Cl－ in the low salt group decreased firstly and then stabilized, and those in the high salt group increased firstly and then stabilized. The qPCR results showed that the CA gene was expressed in all tissues, and the highest expression was found in gill. After salinity stress, the expression level of CA gene mRNA in each experimental group increased and then decreased with time after 24 h, and finally stabilized. The expression levels of CA mRNA in salinity 5 and salinity 35 groups were more obvious than those in salinity 15 and salinity 25 groups.The results showed that the concentrations of Na+, K+ and Cl－ in the blood and the CA gene changed under a salinity stress, adjusted to regulate the ion concentration in vitro and in vivo to maintain the balance of osmotic pressure.The role of ion concentration and CA gene in acute salinity stress provided basic data for the molecular physiological mechanism of Sinonovaulaconstricta in adapting to changes in salinity environment.

Key words: salinity, ion concentration, carbonic anhydrase, Sinonovaulaconstricta

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