生物学杂志

生物学杂志 ›› 2020, Vol. 37 ›› Issue (3): 1-.doi: 10.3969/j.issn.2095-1736.2020.03.001

• 研究报告 •    下一篇

肺癌细胞外泌体GPI-锚定蛋白组学研究

  

  1. 1. 西南交通大学 生命科学与工程学院， 成都 610036； 2. 同济大学 医学院， 上海 200082
  • 出版日期:2020-06-18 发布日期:2020-06-10
  • 通讯作者: 廖海，博士，副教授，研究方向为药用植物学和合成生物学，E-mail：ddliaohai@home.swjtu.edu.cn； 周大鹏，博士，教授，研究方向为糖生物学和免疫治疗，E-mail:dapengzhoulab@tongji.edu.cn
  • 作者简介:吴微波,硕士研究生，研究方向为蛋白组学和糖生物学，E-mail:wuweibo0323@loulook.com
  • 基金资助:
    国家自然科学基金(3187040053);中央高校基本科研基金(22120180201) 

The study of GPI-anchored proteomics in lung cancer cell exosomes

  1. 1. School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu 610036; 2. Medical College, Tongji University, Shanghai 200082, China
  • Online:2020-06-18 Published:2020-06-10

PDF (PC)

98

摘要： GPI（glycosylphosphatidylinositol）锚定蛋白是一类由蛋白质、脂质和糖链共价结合的复杂糖复合物，参与免疫识别、细胞通讯、信号转导等多种生理过程。外泌体调节包括免疫反应、肿瘤生长等在内的许多病理生理过程,其表面的GPI锚定蛋白具有很大研究价值。为了找到可作为肺癌诊断标记物的GPI锚定蛋白，利用TCGA(The Cancer Genome Atlas) 数据库，从肺癌病人和健康人的mRNA阵列数据中筛选到114种GPI锚定蛋白，其中43种存在显著表达差异。以细胞膜总蛋白为对照，使用DIA(Data Independent Acquisition)定量技术对NCI-H838人非小细胞肺癌细胞外泌体蛋白进行定量分析，共筛选出15个外泌体GPI锚定蛋白。利用NCI-H838细胞外泌体GPI锚定蛋白数据和肺癌病人体内的GPI锚定蛋白数据一起进行筛选，最终获得MSLN (mesothelin)、CPM (carboxypeptidase M)、SMPDL3B (sphingomyelin phosphodiesterase, acid-like 3B)、HYAL2 (hyaluronoglucosaminidase 2)、EFNA5(ephrin-A5)、RECK(reversion-inducing-cysteine-rich protein with kazal motifs)和GPC1(glypican 1)7种在外泌体高表达，且可作为肺癌诊断标记物的GPI锚定蛋白。

关键词: GPI锚定蛋白, 外泌体；肿瘤诊断；标记物

Abstract: GPI(Glycosylphosphatidylinositol)-anchored proteins are complexes which consist of proteins, lipids and sugar chains. Studies indicate that GPI-anchored proteins are involved in various physiological processes such as immune recognition, cell communication and signal transduction. Exosomes regulate many pathophysiological processes including immune response and tumor growth, which make it significant to research the GPI-anchored proteins over the exosomes. In order to find the GPI-anchored proteins which can be used as the marker of the detection of lung cancer, 114 GPI-anchored proteins, 43 of which are significantly different between lung cancer patients and healthy people were studied. And the data were screened out using the mRNA expression array data of lung cancer patients and healthy people in TCGA (The Cancer Genome Atlas) database. DIA (Data Independent Acquisition) quantitative and Semi-quantitative technology were used to analysis of proteins from exosomes of NCI-H838 non-small cell lung cancer cell line, with total cellular membrane proteins as control, and 15 GPI-anchored proteins from exosomes were screened out. At last, 7 GPI-anchored proteins, including MSLN (mesothelin), CPM (carboxypeptidase M), SMPDL3B(sphingomyelin phosphodiesterase, acid-like 3B), HYAL2 (hyaluronoglucosaminidase 2), EFNA5(ephrin-A5), RECK(reversion-inducing-cysteine-rich protein with kazal motifs) and GPC1(glypican 1), were searched out by the data of GPI-anchored proteins in lung cancer patients and NCI-H838 cell exosomes, which can be used as the markers in the detection of lung cancer.

Key words: GPI-anchored proteins, exosome, the detection of tumor, marker

中图分类号: