生物学杂志

生物学杂志 ›› 2020, Vol. 37 ›› Issue (1): 72-.doi: 10.3969/j.issn.2095-1736.2020.01.072

• 研究报告 • 上一篇    下一篇

一株野生抗铜大型真菌对铜离子的吸附作用

  

  1. 1. 安庆师范大学 生命科学学院， 安庆 246133； 2. 安庆师范大学 水生生物保护与水生态修复安徽省高校工程技术研究中心, 安庆 246133
  • 出版日期:2020-02-18 发布日期:2020-03-09
  • 作者简介:郑爱芳，硕士，副教授，研究方向为环境微生物学，E-mail: 94198109@qq.com
  • 基金资助:
    安徽省教育厅自然科学基金（KJ2013B122）；安庆师范大学青年基金项目(K050000000124)；安徽省水生生物保护与水生态修复创新团队项目（070-185003）

The copper ion biosorption of a wild macrofungi with copper resistance

  1. 1. College of Life Science, Anqing Normal University, Anqing 246133; 2. Research Center of Aquatic Organism Conservation and Water Ecosystem Restoration in Anhui Province, Anqing Normal University, Anqing 246133, China
  • Online:2020-02-18 Published:2020-03-09

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摘要： 利用平板法筛选出一株具有较强抗铜能力的大型真菌LGBS，研究了菌丝球投加量、pH值、温度、转速、初始铜离子浓度、吸附时间等因素对菌株LGBS在液体条件下生物吸附Cu2+ 的影响。结果显示：实验菌株能够在Cu2+ 浓度低于600 mg/L的PDA平板上生长，经ITS 序列测定，鉴定为白耙齿菌（Irpex lacteus）。当菌丝球投加量从0.5 g/L增加到2.5 g/L时，吸附容量由8.33 mg/g降至3.40 mg/g，而吸附率由20.8%上升到42.5%。在pH 4时，菌体的吸附容量最高；温度为15 ℃ 时吸附效果最好，温度增加对吸附的效果影响不大。转速为50 r/min时，吸附效果最好；初始Cu2+ 浓度由20 mg/L提高为80 mg/L时，吸附容量由3.87 mg/g增至17.21 mg/g；吸附时间为60 min吸附容量最高。研究表明，白耙齿菌菌株LGBS具有较强的吸附重金属Cu2+ 的能力，具有作为重金属吸附剂的潜力。

关键词: 大型真菌, 重金属污染, 铜离子, 生物吸附, 白耙齿菌

Abstract: A macrofungi strain LGBS with high copper resistance was screened by plate method.The species was identified by ITS sequencing analysis. Some factors were studied to analyze the biosorption effects, such as dosages of mycelial pellet, pH, temperature, revolutions per minute( r/min), adsroption time and initial Cu2+ concentrations. The results showed that the ITS sequence of the strain LGBS had a high similarity to Irpex lacteus. It was able to grow on the PDA plates with the initial Cu2+ concentrations under 600 mg/L. The adsorption quantity had a decreasing tendency when the dosages of mycelial pellet was increased. The dosages of mycelial pellet was increased from 0.5 to 2.5 g/L, adsorption quantity decreased from 8.33 to 3.40 mg/g, adsorption rate rised from 20.8% to 42.5%. When pH was 4, the strain had the highest adsorption quantity, and at 15℃, it had the best performance of adsorption; the adsorption quantity decreased slightly as temperatures rose; but if the temperature continued to rise, it had little influence on the adsorption quantity. Under 50 r/min, it had a good adsorption property. The adsorption quantity of Cu2+ increased from 3.87 to 17.21 mg /L with the increasing initial Cu2+ concentrations from 20 to 80 mg /L. The best adsroption time was 60 min, and extending the adsroption time could not enhance the adsorption quantity. The study indicated that Irpex lacteus LGBS has high adsorbability on Cu2+, having bright prospects in future research and application.

Key words: macrofungi, heavy metal pollution, Cu2+, biosorption, Irpex lacteus

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