生物学杂志

生物学杂志

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基于线粒体基因分析黑颈鹤和灰鹤的遗传差异

  

  1. 1. 贵州师范大学 生命科学学院 植物生理与发育调控重点实验室, 贵阳 550025;2. 贵州大学生物多样性与自然保护研究中心, 贵阳 550001
  • 出版日期:2018-12-18 发布日期:2018-12-18
  • 通讯作者: 粟海军，副教授，硕士生导师，研究方向为野生动物资源保护与利用，E-mail：hjsu@gzu.edu.cn
  • 作者简介:王野影，讲师，研究方向为野生动植物保护，E-mail：Wangyeying0818@163.com
  • 基金资助:
    国家自然科学基金项目(31400353);贵州省科技计划重大专项(黔科合重大专项字\[2016\]3022-1号);贵州省重点实验室建设项目(黔科合Z字\[2011\]4005号);教育部长江学者和创新团队发展计划项目(IRT1227);贵州省教育厅自然科学研究项目(黔教合KY字\[2015\]354号);贵州省留学人员科技创新项目(黔人项目资助合同（2016）18号);贵州省科技计划项目(黔科合基础\[2017\]1129；黔科合LH字\[2014\]7682);贵州师范大学资助博士科研项目(0516008) 

The analysis of genetic variation between black-necked crane and common crane based on mitochondrial genes

  1. 1. Key Laboratory of Plant Physiology and Development Regulation, School of Life Science, Guizhou Normal University, Guiyang 550025; 2. Biodiversity and Nature Conservation Research Center, Guizhou University, Guiyang 550001, China
  • Online:2018-12-18 Published:2018-12-18

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摘要： 种群的遗传差异和遗传结构是确定保护等级和保护管理单元的基础，采用黑颈鹤和灰鹤的粪便作为DNA样品来源。通过对粪便DNA进行COI基因的扩增，结合从GenBank上下载的两种鹤的COI序列，对其进行遗传差异分析，发现黑颈鹤和灰鹤群体的COI基因变异很小，几乎为零，建议加强保护力度。通过对两种鹤的线粒体基因组各基因的变异位点比例分析，发现CR 和Cytb两个基因变异位点所占比例相对于其他基因较高，考虑作为黑颈鹤和灰鹤种群遗传学研究的最优分子标记。研究结果将为黑颈鹤和灰鹤保护策略的制定提供科学依据，为鹤属分子系统学和分子粪便学研究奠定基础。

关键词: 黑颈鹤, 灰鹤, 遗传差异, COI, 线粒体基因组

Abstract: It is the genetic variation and structure of population that as the basis to determine the level and the unit of protection. In this research, source of DNA was sampled from faeces of the black-necked crane and common crane. We did the amplification of COI gene of the two cranes, then we analyzed the genetic variance of COI sequences combined with same genes from GenBank. The results showed that COI gene variants in the population of these two cranes are very small, almost zero, so we suggest to strengthen the protection of them. Alternatively, by the analysis of mutation loci ratio for each gene in the mitochondrial genome of black-necked crane and common crane, we found that proportion of mutation loci in CR and Cytb is higher than in other genes. Thus, we considered these four genes are the optimal molecular markers in the population genetics studies of black-necked crane and common crane. These results will provide the basis for the establishment of scientific protection strategy and lay the foundation for the molecular phylogeny and molecular molecular scatology of these two cranes.

Key words: black-necked crane, common crane, genetic diversity, COI, mitochondrial genome

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